We narrowed to 5,142 results for: mos

1. Desmoplakin I tension sensor (F40-based)

   Plasmid

   #119186

   Purpose

   The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYLCRISPR/Cas9P35S-N

   Plasmid

   #66191

   Purpose

   expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   cauliflower mosaic virus 35S promoter (P35S)

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV2ss-U6-sgHTT1-7sk-sgCas9

   Plasmid

   #190900

   Purpose

   AAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAs

   Depositor

   Nicole Déglon

   Article

   Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

   Insert

   sgRNA (HTT spCas9, Human)
   

   Use

   AAV and CRISPR

   Promoter

   U6 and 7sk

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYLCRISPR/Cas9P35S-H

   Plasmid

   #66189

   Purpose

   expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   cauliflower mosaic virus 35S promoter (P35S)

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

   Plasmid

   #86233

   Purpose

   Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   osTir1
   

   Use

   Mouse Targeting

   Tags

   V5 tag

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-DiCas7-11-His

   Plasmid

   #234482

   Purpose

   Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

   Plasmid

   #224497

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting

   Plasmid

   #92141

   Purpose

   Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   osTir1
   

   Use

   Mouse Targeting

   Tags

   V5 tag

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGT9054

   Plasmid

   #219430

   Purpose

   Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS

   Depositor

   Tom Schreiber

   Article

   Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

   Insert

   LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
   

   Use

   CRISPR; Moclo compatible level 1 module (position…

   Tags

   PapE-4LF2

   Expression

   Plant

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRetroX GFP Mps1 wt

    Plasmid

    #63702

    Purpose

    Inducible expression of GFP-Mps1 fusion in mammalian cells

    Depositor

    Floris Foijer

    Article

    Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    Mps1 (TTK Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid

    #217801

    Purpose

    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid

    #208956

    Purpose

    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

    Plasmid

    #208957

    Purpose

    A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid

    #208946

    Purpose

    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherryC1 VAP-B KD/MD

    Plasmid

    #226410

    Purpose

    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    VAP-B (VAPB Human)
    

    Tags

    HA, T7-Xpress tag, and mCherry

    Expression

    Mammalian

    Mutation

    K87D/M89D mutant, unable to bind FFAT motifs

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

    Plasmid

    #211115

    Purpose

    For tagging the C-term of Top2A with Venus

    Depositor

    Job Dekker

    Article

    Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

    Inserts

    5' C-term Top2A homology arm (TOP2A Human)
    Venus
    T2A
    Blastocidin Resistance
    5' C-term Top2A homology arm (TOP2A Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    n/a

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

    Plasmid

    #217804

    Purpose

    Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-linker-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9-NUP98a_IDR-EGFP

    Plasmid

    #232766

    Purpose

    Expresses dCas9-NUP98a_IDR-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-NUP98a_IDR-EGFP (NUP98 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p_sc-eVIP

    Plasmid

    #168174

    Purpose

    Expresses barcoded coding genetic variants in mammalian cells

    Depositor

    Jesse Boehm, James T. Neal, Aviv Regev

    Article

    Ursu et al Nat Biotechnol. 2022 Jun;40(6):896-905.

    Insert

    TP53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    EF-1a

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-FLAG-TAPBPR-TM-VC

    Plasmid

    #135499

    Purpose

    Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Tags

    Luminal/Extracellular FLAG epitope tag, Signal pe…

    Expression

    Mammalian

    Mutation

    switched transmembrane domain with that of MHC-I

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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