We narrowed to 25,030 results for: Nes

1. pENTR221-sGFP

   Plasmid

   #79508

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   superGFP
   

   Use

   Multisite gateway entry clone

   Tags

   6xHis

   Expression

   Mutation

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC-1-AtU6-Y

   Plasmid

   #197779

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   AtU6 promoter for sgRNA assembly
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMC-1-OsU6-X

   Plasmid

   #197780

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   OsU6 promoter for sgRNA assembly
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-Z-SpCas9-gRNA-repeat-polyT-11

   Plasmid

   #197781

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   gRNA repeat for U6 sgRNA assembly
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p1DT (chicken beta 1 integrin)

   Plasmid

   #14066

   Depositor

   Richard Hynes

   Article

   Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

   Insert

   Chicken Beta 1 Integrin (ITGB1 Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pYFP (GB0053)

   Plasmid

   #68197

   Purpose

   Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   YFP coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMaCTag-P01

   Plasmid

   #120012

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   moxBFP
   

   Use

   Pcr template

   Tags

   moxBFP

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nano-lantern(Ca2+)_117/pcDNA3

   Plasmid

   #51985

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-2GS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMC-1-CmYLCV-6

   Plasmid

   #197699

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   CmYLCV promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC-6-RLuc-9

    Plasmid

    #197739

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Renilla luciferase (tomato optimized)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3_omega2

    Plasmid

    #68239

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLIK TT 3xFLAG Luciferase neo

    Plasmid

    #98392

    Purpose

    Lentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6-G6-mCherry

    Plasmid

    #66533

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter G6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - G6

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMaCTag-P32

    Plasmid

    #120043

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    S-Tag
    

    Use

    Pcr template

    Tags

    S-Tag

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330A_D10A-1x3

    Plasmid

    #58773

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMC-1-CmYLCV-4

    Plasmid

    #197694

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    CmYLCV promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-Z25

    Plasmid

    #120068

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    YFP-LID
    

    Use

    Pcr template

    Tags

    YFP-LID

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

    Plasmid

    #133334

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    constitutive

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLIK 3XFLAG-MKK4-EE-NLS hygro

    Plasmid

    #87779

    Purpose

    Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK4
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pMaCTag-P08

    Plasmid

    #120019

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mClover3
    

    Use

    Pcr template

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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