We narrowed to 39,931 results for: lat

1. pcDNA3.1(+)- e18 LOF

   Plasmid

   #131729

   Purpose

   Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-4EBP1 F113A

   Plasmid

   #81122

   Purpose

   expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

   Insert

   4EBP1 F113A (Eif4ebp1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F113A mutation

   Promoter

   CAG

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJZC33

   Plasmid

   #62330

   Purpose

   sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 2x MS2 binding module
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR KIF17 (1-738)

   Plasmid

   #60406

   Purpose

   Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   KIF17 (KIF17 Human)
   

   Use

   Lentiviral

   Tags

   GB1, Strep-Tag, and sfGFP

   Expression

   Mammalian

   Mutation

   Truncated version amino acids 1-738

   Promoter

   SFFV

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX neo PFKP-GFP K281R

   Plasmid

   #138288

   Purpose

   Mammalian Expression, Lentiviral. Expression of human phosphofructokinase platelet isoform PFKP-GFP fusion protein with K281R mutation

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   PFKP (PFKP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K281R

   Promoter

   CMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9-EF-5Pou5f1

   Plasmid

   #174872

   Purpose

   CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PPP1CA

   Plasmid

   #155843

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PPP1CA (PPP1CA Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH_TNF-SBP-mCherry

   Plasmid

   #65283

   Purpose

   TNF reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQFlag-USP11 CS puroR

   Plasmid

   #46748

   Purpose

   Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C318S--catalytically inactive

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPPC011

    Plasmid

    #171143

    Purpose

    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Sp.pCas9, BBa_J23107

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. hnRNPA2_LC_nocharge

    Plasmid

    #139118

    Purpose

    express hnRNPA2 LC with no charged residues

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all charged residues are changed to S/Q except fo…

    Promoter

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS5-3HA/p14ARF

    Plasmid

    #78764

    Purpose

    To overexpress p14ARF in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    p14ARF (CDKN2A Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pRL-TK 3'FBXW7 UTR

    Plasmid

    #26649

    Depositor

    Alex Minella

    Article

    Xu et al J Biol Chem. 2010 Nov 5;285(45):34439-46

    Insert

    FBXW7 (FBXW7 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains only the 3'UTR of FBXW7, nucleotide…

    Promoter

    Available Since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pJZC48

    Plasmid

    #62336

    Purpose

    sgRNA + 1x COM with COM-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x COM binding module
    COM-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. S142D drebrin-YFP

    Plasmid

    #58336

    Purpose

    mammalian expression of human drebrin-YFP phospho-mimetic mutant

    Depositor

    Phillip Gordon-Weeks

    Article

    Worth et al J Cell Biol. 2013 Sep 2;202(5):793-806.

    Insert

    drebrin (DBN1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    S142D, phospho-mimetic mutant

    Promoter

    CMV

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

16. S142A drebrin-YFP

    Plasmid

    #58335

    Purpose

    mammalian expression of human drebrin-YFP phospho-dead mutant

    Depositor

    Phillip Gordon-Weeks

    Article

    Worth et al J Cell Biol. 2013 Sep 2;202(5):793-806.

    Insert

    drebrin (DBN1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    S142A, phospho-dead mutant

    Promoter

    CMV

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

17. F2-bio-His

    Plasmid

    #52179

    Purpose

    Expresses full-length Prothrombin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    F2 (F2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pClneoMyc human MST2

    Plasmid

    #37022

    Depositor

    Yutaka Hata

    Article

    Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

    Insert

    MST2 (STK3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

19. PDPN-bio-His

    Plasmid

    #52313

    Purpose

    Expresses full-length Podoplanin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PDPN (PDPN Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3 T7 DLC1

    Plasmid

    #24266

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    DLC1 (DYNLL1, DLC1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only