Addgene

We narrowed to 35,854 results for: REP

Showing: 3821 - 3840 of 35854 results

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His6_MBP_FMRP_P451S

Plasmid

#198693

Purpose

Expression of human FMRP protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FMR1 (FMR1 Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

changed proline 451 to serine

Promoter

T7

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMK287 (mAID-Hygro)

Plasmid

#72825

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Hygro

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee

Plasmid

#140836

Purpose

mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pXJ-Nanoluc (Nluc)

Plasmid

#232656

Purpose

Nanoluc luciferase expression driven CMV promoter

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

Nanoluc

Use

Luciferase

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-p65-AD

Plasmid

#138395

Purpose

RMCE vector for generating C-terminal T2A-p65-AD fusion (split GAL4)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-p65-AD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-GAL4-DBD

Plasmid

#138394

Purpose

RMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4-DBD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-LAMP1-EGFP

Plasmid

#169003

Purpose

Express rat LAMP1-EGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

Insert

rat LAMP1-EGFP

Use

Unspecified

Tags

EGFP

Expression

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCX-mutSpiCee

Plasmid

#140903

Purpose

mammalian expression of a mutated form of SpiCee, that does not affect calcium signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

mutSpiCee

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-mRuby3

Plasmid

#74252

Purpose

Mammalian expression of tandem fusion of mClover3-mRuby3

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mClover3-mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pF3BGX-7xGFP11

Plasmid

#138397

Purpose

RMCE vector for generating C-terminal fusion of seven tandem GFP11 tags (split GFP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

7xGFP11

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMBA334

Plasmid

#214746

Purpose

High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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PGK 4xMito-mEmerald

Plasmid

#200430

Purpose

Mammalian expression of fluorescently-tagged marker for mitochondria

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence

Use

Tags

mEmerald

Expression

Mammalian

Mutation

4x mito-targeting sequence

Promoter

PGK

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 Wt (1-674aa)

Plasmid

#75478

Purpose

mammalian expression of GADD34

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pET32a-hTGFa

Plasmid

#199236

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Transforming growth factor alpha, partial (TGFA Human)

Use

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pKeratin-Electra2

Plasmid

#184928

Purpose

Keratin labeling with blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Keratin-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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EC2_4_dCas9_VPR_sgRNA

Plasmid

#163709

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Use

Tags

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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MmXYRS-4xM15

Plasmid

#155343

Purpose

Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.

Depositor

Irene Coin

Article

Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

Insert

Humanized MmXYRS/tRNAM15

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/U6

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pXCas9H840A

Plasmid

#60900

Purpose

Dual Expression Vector for Cas9 H840A nickase and gRNA

Depositor

Bruce Conklin

Article

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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PARP2-c098

Plasmid

#216873

Purpose

Bacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

Insert

PARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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