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pYTK066

Plasmid

#65173

Purpose

Encodes tTDH1 as a Type 4b part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

tTDH1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVS_AF

Plasmid

#188950

Purpose

Level 2 destination vector containing pBBR1 origin of replication and CIDAR AF scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK081

Plasmid

#65188

Purpose

Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

CEN6/ARS4

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK089

Plasmid

#65196

Purpose

Encodes AmpR-ColE1 as a Type 8a part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

AmpR-ColE1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK076

Plasmid

#65183

Purpose

Encodes HIS3 as a Type 6 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

HIS3

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK047

Plasmid

#65154

Purpose

Encodes GFP dropout as a Type 234r part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

GFP dropout

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVC_AG

Plasmid

#188954

Purpose

Level 2 destination vector containing pBBR1 origin of replication and CIDAR AG scars

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIT5_SL

Plasmid

#173769

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK036

Plasmid

#65143

Purpose

Encodes Cas9 as a Type 3 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

Cas9

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199486

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pIT5_KT

Plasmid

#173773

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_KH

Plasmid

#173772

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199485

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS279

Plasmid

#73729

Purpose

SapTrap FLP-on C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

C-terminal FLP-on

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pYTK035

Plasmid

#65142

Purpose

Encodes I-SceI (ORF) as a Type 3 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

I-SceI (ORF)

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK014

Plasmid

#65121

Purpose

Encodes pTEF2 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pTEF2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK012

Plasmid

#65119

Purpose

Encodes pHHF2 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pHHF2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK019

Plasmid

#65126

Purpose

Encodes pPAB1 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pPAB1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK010

Plasmid

#65117

Purpose

Encodes pCCW12 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pCCW12

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pIT5_CL

Plasmid

#173767

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge