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pPN107

Plasmid

#91636

Purpose

Express sgRNA targeting human KDM4A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KDM4A (KDM4A Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN106

Plasmid

#91635

Purpose

Express sgRNA targeting human KDM4A

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KDM4A (KDM4A Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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Thy-Kv2.1-HA

Plasmid

#111541

Purpose

For expression of human KCNB1 in mouse brain

Depositor

Federico Sesti

Article

Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

Insert

thy1.2-Kv2.1-HA (KCNB1 Human)

Use

Mouse Targeting

Tags

HA

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-CMV-Cdc42 3'UTR mutant (position 577-599)

Plasmid

#51391

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

NM_001039802.1 (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Mutation

miR-29 binding site mutant (position 577-599)

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pCNL-C1_Rab11a

Plasmid

#65706

Purpose

Expresses CNL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-S320C

Plasmid

#45702

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

S320C

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Gp88 in situ probe

Plasmid

#45628

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Gpr88 in situ probe (Gpr88 Mouse)

Use

In situ

Mutation

fragment contains bp# 2742-3255 of AB042408.1 or …

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pSP64-hERG1a F656A

Plasmid

#53054

Purpose

Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Phenylalanine 656 to Alanine

Promoter

SP6

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a Y652A

Plasmid

#53053

Purpose

Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Tyrosine 652 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pZF-AAA

Plasmid

#127465

Purpose

coding sequence for wild type zinc finger ZF-AAA (ZiF268)

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-AAA (EGR1 Human)

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Tubulin-6

Plasmid

#55097

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pX61

Plasmid

#46852

Purpose

TOP Hupki-puro2A-Hupki-wt plasmid

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 wild type (TP53 Mouse, Human)

Expression

Mammalian

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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mPapaya-Actin-C-18

Plasmid

#56395

Purpose

Localization: Actin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Actin (ACTB Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAG175 AK024929 3'UTR mut

Plasmid

#12033

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N8 3'UTR mut (LINC00341 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pBMN GBRP

Plasmid

#89293

Purpose

For expression of the mammalian Atg8 protein GABARAP without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAP (GABARAP Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2c-K660E

Plasmid

#45706

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660E

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pAP1791-1

Plasmid

#105246

Purpose

human eGFP with homology arms for RAB11A (recoded )

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with homology arms for RAB11A (recoded *) (RAB11A Human)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pPN269

Plasmid

#91632

Purpose

Express sgRNA targeting human KCNB1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

KCNB1 (KCNB1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 neo

Plasmid

#72569

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2c-T787K

Plasmid

#45710

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

T787K

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only