We narrowed to 11,549 results for: ANG

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

Plasmid

#107730

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

Plasmid

#107729

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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sgALK(A)

Plasmid

#85562

Purpose

Expresses sgRNA targeting human ALK around codon F1174

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

anaplastic lymphoma receptor tyrosine kinase (ALK Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pLJM1-FLAG-Sestrin2-E451A

Plasmid

#73677

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pLJM1-FLAG-Sestrin2-L261A

Plasmid

#73676

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

Plasmid

#69227

Purpose

Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLK15

Plasmid

#37253

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

Scribble (SCRIB Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 382-1630

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA6-rbN1 S77A

Plasmid

#32945

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

SLc9a3r1 (SLC9A3R1 rabbit)

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Change Serine77 to Alanine

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Claspin-BPII

Plasmid

#31632

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

aa 492-622 of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
p203 Dync1li1 N235Y

Plasmid

#26451

Depositor

Elizabeth Fisher

Insert

cytoplasmic dynein light intermediate chain 1 N235Y (Dync1li1 Mouse)

Use

Subcloning and sequencing

Mutation

changed Asparagine 235 to Tyrosine

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2Q545AK570A

Plasmid

#14105

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Gln545 to Ala and Lys570 to Ala

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
Syn3F31A

Plasmid

#13398

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. F31 of s…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
Syn3D33A

Plasmid

#13400

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. D33 of s…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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sup35-C653R

Plasmid

#1198

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Expression

Yeast

Mutation

changed cys 653 to arg

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pInducer20 ALPK1 T237M

Plasmid

#231586

Purpose

ALPK1 gene mutated in position T237M (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

changed threonine 237 to Methionine

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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SGLT1(GFP)-MAP17(nb)

Plasmid

#216210

Purpose

for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-PLA2G15-6xHis S198A

Plasmid

#213605

Purpose

Expresses PLA2G15-6xHis S198A in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

Phospholipase A2 Group XV (PLA2G15 Human)

Tags

6xHis

Expression

Mammalian

Mutation

Changed serine 198 to alanine

Promoter

CMV

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV WT SPOP 28-359

Plasmid

#194115

Purpose

Wild type His-SUMO SPOP expression plasmid

Depositor

Tanja Mittag

Article

Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

Insert

SPOP (SPOP Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCjedCas9

Plasmid

#172216

Purpose

Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells

Depositor

John van der Oost

Article

Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

Insert

catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system

Tags

Histag

Mutation

changed Asp 8 to Ala and His 559 to Ala

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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EC71022

Plasmid

#159608

Purpose

Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

loxP-mCherry-HDEL-t35S-loxP

Use

Cre/Lox and Synthetic Biology

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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