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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 3861 - 3880 of 12282 results

  1. pGBW-m4134229

    Plasmid
    #152097
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152097-plasmid-map-sequence-id-297924

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  5. Clomeleon

    Plasmid
    #203723
    Purpose
    A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration
    Depositor
    George Augustine
    Publication
    Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cyan fluorescent protein linked yellow florescent protein
    Expression
    Bacterial and Mammalian
    Mutation
    Cyan Fluorescent Protein, Topaz Fluorescent Prote…
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203723-plasmid-map-sequence-id-403632

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  6. pCAGGS-SARS2-S-FKO (NC-flag)

    Plasmid
    #159363
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.
    Depositor
    Hyeryun Choe, Michael Farzan
    Publication
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S protein (S SARS-CoV-2)
    Tags
    Flag and Flag
    Expression
    Mammalian
    Mutation
    Furin-cleavage site mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159363-plasmid-map-sequence-id-310910

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  7. pGBW-m4133784

    Plasmid
    #152558
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152558-plasmid-map-sequence-id-317273

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  8. pGBW-m4046538

    Plasmid
    #149128
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    May 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149128-plasmid-map-sequence-id-296274

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  11. pcDNA-NES-ZapCmR1.1

    Plasmid
    #59014
    Purpose
    Cytosolic zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
    Tags
    Nuclear Exclusion Signal
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. PB-NES-ZapCV2-Zinc dead

    Plasmid
    #203850
    Purpose
    Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells
    Depositor
    Amy Palmer
    Publication
    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZapCV2-Ala zinc dead sensor
    Tags
    Nuclear Export Signal
    Expression
    Mammalian
    Mutation
    Cys and His in Zap1 is mutated with Ala
    Promoter
    CMV promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    203850-plasmid-map-sequence-id-402879

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  15. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid
    #132962
    Purpose
    Firefly-luciferase single-strand annealing assay cloning backbone
    Depositor
    Gang Bao
    Publication
    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Firefly Luciferase
    Use
    CRISPR and Luciferase
    Expression
    Mammalian
    Mutation
    Stop sequence between luciferase gene
    Promoter
    CMV
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    132962-plasmid-map-sequence-id-259217

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  18. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid
    #208035
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208035-plasmid-map-sequence-id-410209

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  19. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid
    #208963
    Purpose
    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EcKlenow-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208963-plasmid-map-sequence-id-415798

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  20. pcDNA3.1-VEGFR3-V5/HIS

    Plasmid
    #205618
    Purpose
    Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VEGFR3 receptor tyrosine kinase (FLT4 Human)
    Tags
    V5-His
    Expression
    Mammalian
    Mutation
    T28M, S455R- Please see depositor comment
    Promoter
    CMV
    Available since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205618-plasmid-map-sequence-id-407177

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Showing: 3861 - 3880 of 12282 results