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pTRIP-CMV-Puro-2A

Plasmid

#102611

Purpose

Control lentivector expressing Puro resistance and gene of interest from CMV promoter

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003

Plasmid

#133566

Purpose

Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

EGFP-Talin head-SpyCatcher003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pTfR-PAmKate

Plasmid

#32692

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Tags

transferrin receptor (TfR)

Expression

Mammalian

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

CMV

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPig_8X-TOPFLash-tdIRFP_Puro

Plasmid

#249713

Purpose

TopFlash reporter with puromycin. Used as a live readout of downstream Wnt gene transcription.

Depositor

Max Wilson

Article

Rosen et al bioRxiv [Preprint]. 2025 May 23:2025.02.

Inserts

tdiRFP
T2A-PuroR

Expression

Mammalian

Promoter

TopFlash and TopFlash, with 7x TCF/LEF motif

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP

Plasmid

#157733

Purpose

mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::rtTA3-bGHpA

Plasmid

#62445

Purpose

MXS_chaining vector with CMV::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p415-GalL-Cas9-CYC1t

Plasmid

#43804

Purpose

A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

Human Optimized S. pyogenes Cas9

Use

CRISPR

Expression

Yeast

Promoter

GalL

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-NCre

Plasmid

#106368

Purpose

N-terminal part of the split-Cre System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

Insert

NCre

Use

Cre/Lox

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPEG

Plasmid

#162466

Purpose

Entry vector for gRNA insertion in plant prime editing expression vector

Depositor

Adam Bogdanove

Article

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Type

Empty backbone

Use

Entry vector for grna

Promoter

CmYLCV

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

Plasmid

#169097

Purpose

Cas9 and Citrine expression in transfected cells

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

Cas9-2A-Citrine

Expression

Mammalian

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag3B-CCre

Plasmid

#106369

Purpose

C-terminal part of the split-Cre System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

Insert

CCre

Use

Cre/Lox

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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CBla_2.0_EmrE_TMD4_3P

Plasmid

#207848

Purpose

BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

C-BLa 2.0 fusion protein

Tags

Flag (DYKDHDG)

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag003-Talin rod-mCherry

Plasmid

#133567

Purpose

Expresses SpyTag003-Talin rod-mCherry in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-Talin rod-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cyto-CaNARi

Plasmid

#64849

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Tags

Cerulean, Nuclear export signal (NES), and Venus

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSEM253 - sgRNA mosTI hygroR

Plasmid

#159827

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI hygroR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM270 - MCS mosTI hygroR target

Plasmid

#159826

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI hygroR target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV

Plasmid

#235547

Purpose

To express GFP

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Type

Empty backbone

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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