We narrowed to 10,107 results for: transfer
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Plasmid#162379PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pErCas12a EZ Clone
Plasmid#132641PurposeExpresses ErCas12a and an sgRNA to target a region of interestDepositorInsertErCas12a
UseAAV and CRISPRTags3x HA and SV40 NLSExpressionMammalianMutationQ926R (Please see depositor comments) and BsaI si…PromoterCMV PromoterAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC475 - pAAV c-fos iRFP
Plasmid#47906PurposeAn AAV vector that expresses iRFP under the c-fos promoter.DepositorInsertInfrared fluorescent protein
UseAAVExpressionMammalianPromoterc-fosAvailable SinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-SOUL-P2A-tdTomato-WPRE-BGHpA
Plasmid#177577PurposeExpresses SOUL in Cre-positive cellsDepositorInsertSOUL
UseAAVTagstdTomatoPromoterEF1aAvailable SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.A184S
Plasmid#106174PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1 and AAV9InsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterhSynapsinAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-GCaMP6f
Plasmid#160722PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-GCaMP6f
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19
Plasmid#111741PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ19 (polyQ repeat)PromoterCMV and P10Available SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79
Plasmid#111729PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ79 (polyQ repeat)PromoterCMV and P10Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ASAP3-WPRE
Plasmid#132331Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vectorDepositorInsertASAP3
UseAAVExpressionMammalianPromoterhSynapsinAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23
Plasmid#111726PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ23 (polyQ repeat)PromoterCMV and P10Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hAdipoq-GFP
Plasmid#176215PurposeExpresses GFP under the control of distal enhancer and the promoter of human adiponectin geneDepositorInsertGFP
UseAAVExpressionMammalianAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FlpE-bGHpA
Plasmid#50364PurposeCan be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoterDepositorInsertFlpE recombinase gene
UseAAVPromoterhSyn1Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36
Plasmid#111745PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ36 (polyQ repeat)PromoterCMV and P10Available SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3Kv-WPRE
Plasmid#132330Purposeexpresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3KV
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-HTR6-SNAPf-TRIP
Plasmid#189617PurposeExpresses cilia-targeted RhoA inhibitory peptide, tet-inducibleDepositorInsertHTR6-TRIP_alpha
UseAAVTagsalphaTag in the N-term of HTR6, SNAP-tag in the C…PromoterTREAvailable SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-[EF1α-CD19 4-1BB]
Plasmid#218336PurposeLentiviral transfer plasmids for mammalian expression CD19 CARDepositorInsertCD19 CAR mammalian expression cassette
UseLentiviralExpressionMammalianAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN-[EF1α-CD19 4-1BB]
Plasmid#218340PurposeLentiviral transfer plasmids for mammalian expression CD19 CARDepositorInsertCD19 CAR mammalian expression cassette
UseLentiviralExpressionMammalianAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE
Plasmid#162381PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterCAGAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-axon-jYCaMP1s
Plasmid#135421PurposeAxon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variantDepositorHas ServiceAAV1InsertjYCaMP1s
UseAAVTagsGAP43 axon targeting sequenceExpressionMammalianPromoterhSynAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30
Plasmid#111744PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ30 (polyQ repeat)PromoterCMV and P10Available SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pmSyn1-EBFP-Cre
Plasmid#51507PurposeCan be used to generate AAV virus that will express Cre in neurons from the synapsin promoterDepositorHas ServiceAAV5InsertEBFP-Cre
UseAAVPromoterpmSyn1Available SinceJuly 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA
Plasmid#216320PurposeSplit luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Luciferase + splice donor site
UseAAVPromoterCMVAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54
Plasmid#111727PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ54 (polyQ repeat)PromoterCMV and P10Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA
Plasmid#216321PurposeSplit luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Luciferase + splice acceptor site
UseAAVPromoterCMVAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX551-miniCMV-SpCas9D10A nickase
Plasmid#216735PurposeExpresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRExpressionMammalianMutationD10APromoterT7 and mini-CMVAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB-rDA3m
Plasmid#208708PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons in a cre-dependent mannerDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3m
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
DRH337: AAV-hsyn-CheRiff-eGFP
Plasmid#51697PurposeAAV production of CheRiff channelrhodopsin actuatorDepositorInsertCheRiff-eGFP
UseAAVTagseGFPPromoterhSynapsinAvailable SinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE
Plasmid#162382PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterCAGAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA
Plasmid#216318PurposeSplit fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertsplit cerulean fluorescent protein + splice donor site
UseAAVPromoterCMVAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eGFP-uTEV3
Plasmid#234320PurposeMammalian expression of uTEV3 tagged with an eGFP markerDepositorInserteGFP-uTEV3
UseAAVTagseGFPExpressionMammalianMutationMutations respective to the wild type TEV: S219V …PromoterCMVAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only