We narrowed to 5,172 results for: PEP

1. pSCM167.2-Venus-VF-hADAM10(aa455-550)

   Plasmid

   #189556

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 455-550

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSCM167-Venus-VF-hADAM10(aa220-459)

   Plasmid

   #189555

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 220-459

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCM167.2-Venus-VF-hADAM10(aa551-654)

   Plasmid

   #189557

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only cysteine rich domain--amino acids 551-654

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa551-654

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

   Plasmid

   #189562

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   contains aa 1-213

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCM167-Venus-VF-hADAM10(aa455-654)

   Plasmid

   #189561

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 455-654

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GST-SmB'

   Plasmid

   #1842

   Depositor

   Philip Leder

   Article

   Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

   Insert

   SmB' (SNRPB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP ratAPOBEC1 L173A/G227A

   Plasmid

   #167169

   Purpose

   Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

   Depositor

   Silvestro Conticello

   Article

   Chieca et al bioRxiv 2021

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Leucine 173 to Alanine; changed Glycine 2…

   Promoter

   CMV promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOCC121

   Plasmid

   #118893

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCIneo-HA-Ago2

   Plasmid

   #115362

   Purpose

   Expression vector to produce HA-tagged human Ago2 protein without N-peptide

   Depositor

   Witold Filipowicz, Ramesh Pillai

   Article

   Pillai et al RNA. 2004 Oct . 10(10):1518-25.

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCellFree_G03 NFKB1

    Plasmid

    #67126

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFKB1 (NFKB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pQTEV-GTF2F2

    Plasmid

    #31323

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    general transcription factor IIF (GTF2F2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc-­His-­zfTpo

    Plasmid

    #64309

    Purpose

    Express soluble His-tagged zebrafish thrombopoietin in insect cells

    Depositor

    Petr Bartunek

    Article

    Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

    Insert

    Thrombopoietin (thpo Zebrafish)
    

    Use

    Tags

    (His)6

    Expression

    Insect

    Mutation

    Thrombopoietin starts at AA37 (AA1-36 encode the …

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pLyGo-Ec-6

    Plasmid

    #163132

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. rat APOBEC1-EGFP

    Plasmid

    #112857

    Purpose

    plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein

    Depositor

    Silvestro Conticello

    Article

    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

    Insert

    apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PcDNA-DEST47-CypB-Del3 C-term GFP

    Plasmid

    #36136

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 39-216 of cyclophilin B; G63…

    Promoter

    CMV

    Available since

    Nov. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3-HA-human MYCN-V421D

    Plasmid

    #74166

    Purpose

    Mammalian expression of human MycN-VD mutant

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MycN (MYCN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V421D

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pLyGo-Kp-2

    Plasmid

    #163144

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC92

    Plasmid

    #118910

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Use

    Tags

    eGFP-HIS6

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC6

    Plasmid

    #118864

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, EGFP

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge