167,785 results

1. HDAC3 Flag

   Plasmid

   #13819

   Purpose

   Mammalian expression of human histone deacetylase 3 with flag tag

   Depositor

   Eric Verdin

   Article

   Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

   Insert

   HDAC3 (HDAC3 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-UbE2C

   Plasmid

   #15779

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2C (UBE2C Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

   Plasmid

   #201681

   Purpose

   Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

   Depositor

   Emma Rawlins

   Article

   Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

   Inserts

   SFTPC promoter region (2kb upstream)
   TagRFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSFV-Helper1

   Plasmid

   #92073

   Purpose

   This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

   Depositor

   Henrik Garoff

   Article

   Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

   Insert

   cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
   

   Use

   Helper plasmid, for run-off transcription and rna…

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-PSD-95 [K28/43R]

   Plasmid

   #128618

   Purpose

   Mammalian Expression Plasmid of anti-PSD-95 (Human). Derived from hybridoma K28/43.

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   Anti-PSD-95 (Human) recombinant mouse monoclonal antibody (DLG4 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-neo-hCG

   Plasmid

   #16574

   Depositor

   Bert Vogelstein

   Article

   Shih et al Nat Med. 2000 Jun . 6(6):711-4.

   Insert

   Beta-hCG (CGB3 Human)
   

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pBbB19k-GFP

   Plasmid

   #126135

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23105

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

   Plasmid

   #154868

   Purpose

   Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

   Depositor

   Ulrik Gether

   Has Service

   AAV Retrograde and AAV8

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Flp-frt

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Human Synapsin-1

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRB-mCherry-FTH1

   Plasmid

   #100749

   Purpose

   Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   ferritin heavy chain 1 (FTH1 Human)
   

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184741

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    David Schaffer, John Flannery

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Mutation

    7mer insertion in the AAV2 cap

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-NFAT4(3-407)-GFP

    Plasmid

    #21664

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    NFAT4 (NFATC3 Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Amino acids 3-407

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. vsv-Sgo1-GFP

    Plasmid

    #108494

    Purpose

    expression of vsv-Sgo1-EGFP

    Depositor

    Susanne Lens

    Article

    Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

    Insert

    Shugoshin 1 (SGO1 Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA

    Plasmid

    #108747

    Purpose

    Expresses N-terminus to aa27 of Flp recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa27
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTXB1-sfGFP

    Plasmid

    #51562

    Purpose

    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

    Depositor

    Francisco Malagon, Sankar Adhya

    Insert

    superfolder GFP
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3xnls-mScarlet3_N1

    Plasmid

    #189775

    Purpose

    Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    3xnls-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRKH3-ermGFP

    Plasmid

    #27169

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Mutation

    presence of an additional 183-bp region at N term…

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Mm.cargo(Cas9)

    Plasmid

    #174865

    Purpose

    Expresses mouse Peg10 cargoRNA encoding SpCas9

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    SpCas9 flanked by MmPeg10 UTRs
    

    Tags

    HA-SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMA3790

    Plasmid

    #70110

    Purpose

    mtDNA elimination/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    hUNG1 Y147A
    Neo
    

    Tags

    None, human OTC mitochondrial targeting sequence,…

    Expression

    Mammalian

    Mutation

    Y147A, aa's 1-77 removed

    Promoter

    CMV, EF1a, and SV40 and native

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCSG17-NleL

    Plasmid

    #66716

    Purpose

    Expresses NleL (170-782) in E. coli

    Depositor

    David Komander

    Article

    Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

    Insert

    NleL (NEWENTRY )
    

    Tags

    GST-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only