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p104_gRNA_Sp_CD3e

Plasmid

#213780

Purpose

Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sp gRNA

Use

CRISPR and Lentiviral

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNM3755

Plasmid

#154116

Purpose

tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆pes-10::GFP-C1::act-4 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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TU#867 (Punc-119sid-1)

Plasmid

#25811

Depositor

Martin Chalfie

Article

Calixto et al Nat Methods. 2010 Jul;7(7):554-9. doi: 1

Insert

systemic RNA interference-deficient 1 (sid-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pCWori-A13AMO-aaCPRct

Plasmid

#20117

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

Amorphadiene oxidase and p450 reductase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pDEW8

Plasmid

#84238

Purpose

Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

Depositor

David Weinberg

Article

DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

Insert

HA-mRuby-P2A-eGFP

Tags

HA-mRuby and eGFP

Expression

Yeast

Promoter

GPD

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pNM3774

Plasmid

#154117

Purpose

tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆mec-7p::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMIN-Ri

Plasmid

#197784

Purpose

Level 2 destination vector: pMIN-Ri backbone

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJJB1577

Plasmid

#218619

Purpose

Express Pex5, Pex2, Pex17, and Pex10

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP1-VP1-588TAG

Plasmid

#240122

Purpose

Express AAV2 capsid with ncAA mutant at site 588 of VP1

Depositor

Abhishek Chatterjee

Article

Pham et al bioRxiv 2024.07.20.604406

Inserts

RC2-DelVP1
VP1-588-TAG

Use

AAV

Expression

Mammalian

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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DV4 VLPs pVRC8400

Plasmid

#63164

Purpose

DV4 prM-E structural cassette in pVRC8400

Depositor

Stephen Harrison

Article

Chao et al Elife. 2018 Jul 12;7. pii: 36461. doi: 1

Insert

DV4 prM-E

Expression

Mammalian

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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TS245_pNKI-2-13_EGFP-DPN7

Plasmid

#200719

Purpose

producing Methyl Tracer EGFP protein in bacteria

Depositor

Bas van Steensel

Article

van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

Insert

eGFP-DPN7

Tags

6xHis

Expression

Bacterial

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW mEmerald-P2A-NeoR

Plasmid

#239569

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mEmerald-P2A-PuroR

Plasmid

#239566

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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DV3 VLPs pVRC8400

Plasmid

#63163

Purpose

DV3 prM-E structural cassette in pVRC8400

Depositor

Stephen Harrison

Article

Chao et al Elife. 2018 Jul 12;7. pii: 36461. doi: 1

Insert

DV3 prM-E

Expression

Mammalian

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-C1

Plasmid

#31926

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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p105_gRNA_Sa_CD40

Plasmid

#213781

Purpose

Expresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sa gRNA

Use

CRISPR and Lentiviral

Promoter

mU6

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJPC063

Plasmid

#234533

Purpose

TRV2 expressing Int13

Depositor

Daniel Voytas

Article

Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

Insert

Integrase 13 recombinase

Use

Recombinase

Expression

Plant

Promoter

CaMV 35S

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSLfa-PUb-EGFP-3'UTR#13

Plasmid

#176338

Purpose

The synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

Depositor

Zach Adelman

Article

Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

Insert

3'UTR#13

Expression

Insect

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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VV063: 1xCox8 - superecliptic pHluorin in fck

Plasmid

#58500

Purpose

Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pNM3725

Plasmid

#154113

Purpose

UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

UAS 11X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
VV064: 1xCox8 - ratiometric pHluorin in fck

Plasmid

#58502

Purpose

Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEVY-GT

Plasmid

#51231

Purpose

bi-directional expression vector for yeast, galactose inducible, TRP1 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS190

Plasmid

#34977

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb

Expression

Yeast

Promoter

ADH

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pscAAV-CBh-aSyn-WPRE3-enSV40pA

Plasmid

#194244

Purpose

Expresses human alpha-Synuclein WT

Depositor

Michael J Fox Foundation MJFF

Insert

aSynuclein(WT) (SNCA Human)

Use

AAV

Promoter

CBh

Available Since

Jan. 17, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEX Syt1 C2AB (96-421)

Plasmid

#170643

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNM3724

Plasmid

#154112

Purpose

tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSynW Syt1 WT IRES mRuby3

Plasmid

#170639

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJDC182

Plasmid

#60854

Purpose

Expresses firefly luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Firefly luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pDest eGFP CUGBP1

Plasmid

#61276

Purpose

Mammalian expression of EGFP-tagged human CUGBP1

Depositor

Nicolas Charlet-Berguerand

Insert

CUG-BP (CELF1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pScalps hygro

Plasmid

#184785

Purpose

Empty Lentiviral vector backbone

Depositor

Ajit Chande

Article

Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

Type

Empty backbone

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Filamin-C-stFRET

Plasmid

#61107

Purpose

Expression of Filamin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mVenus-PA-MKI

Plasmid

#65457

Purpose

photoactivatable myosin light-chain kinase inihibition

Depositor

Klaus Hahn

Article

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

MKI

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pGL3Basic-Mdm2-T3

Plasmid

#32369

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
SRpHi4

Plasmid

#102544

Purpose

Bacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

EYFP-EGF

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEVY-GA

Plasmid

#51227

Purpose

bi-directional expression vector for yeast, galactose inducible, ADE2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3Basic-Mdm2-T4

Plasmid

#32370

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
SRpHi2

Plasmid

#102542

Purpose

Bacterial expression of EGFP fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-EGFP

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDS244

Plasmid

#34982

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Ste11 (STE11 Budding Yeast)

Expression

Yeast

Promoter

ADH

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
SRpHi3

Plasmid

#102543

Purpose

Bacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purification

Depositor

Stefan Hell

Article

Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

Insert

Tat-sepHluorin

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Filamin-M-stFRET

Plasmid

#61105

Purpose

Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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