We narrowed to 21,023 results for: ache
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Plasmid#161955PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHHDepositorInsertpSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
ExpressionBacterialPromoterPBADAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcry:mCherry,-600unc:cct3GFP
Plasmid#172528PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.DepositorInsertcct3-GFP
ExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gLacZ.EFS-NS.H2B-RFP
Plasmid#170363PurposeNegative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralPromoterEFS-NSAvailable SinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN071
Plasmid#166729PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN072
Plasmid#166730PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1-NLS
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
SaLgCG
Plasmid#164563PurposeGFPDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 promoter for crRNA expression and EFS promoter…Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-C-DiB-RM
Plasmid#168477PurposeC-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertLeucine Zipper + C-fragment of DiB-RM
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-DiB-RM
Plasmid#168475PurposeN-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertN-fragment of DiB-RM + Leucine Zipper
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_ReBphP-PCM_IRES_sfGFP
Plasmid#166917PurposeExpress ReBphP-PCM and sfGFPDepositorInsertsReBphP-PCM
sfGFP
ExpressionMammalianPromoterCMVAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc180
Plasmid#161942PurposeCarries Golden Gate substitution insert for acVHH-assisted bisection mappingDepositorInsertpSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
ExpressionBacterialPromoterPBADAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc226
Plasmid#161944PurposeContains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmidDepositorInsertpSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
ExpressionBacterialPromoterPBADAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 UPA-STOP
Plasmid#164031PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPADepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)
Plasmid#164916PurposeExpresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTRDepositorInsertGCN4(UTR)-ZEM(TF)
UseSynthetic BiologyExpressionYeastPromoterPGK1 (yeast)Available SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
Plasmid#159844PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
Plasmid#159865PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKH-1699
Plasmid#124227PurposeMammalian SpCas9-HF1 expressionDepositorInsertSpCas9-HF1
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA44
Plasmid#158522PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-eGFP. Km plant selection marker.DepositorAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA16
Plasmid#158494PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorMutationL17E, D481V, no STOP codonAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA7
Plasmid#158485PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorMutationL17E, STOP codonAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA15
Plasmid#158493PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorMutationL17E, no STOP codonAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only