We narrowed to 51,047 results for: Ide

1. CMV-LUC2CP/intron/ARE

   Plasmid

   #62858

   Purpose

   splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

   Depositor

   Gideon Dreyfuss

   Article

   Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

   Insert

   Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   insertion of chimeric intron at nueotide poclsiti…

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1_-DIO-GRAB_NPY1.0

   Plasmid

   #208677

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
   

   Use

   AAV

   Promoter

   EF1_

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL7 lenti-dcas9-3xflag-blast

   Plasmid

   #112133

   Purpose

   lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   dCas9(D10A, N863A)-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A mutants in Cas9

   Promoter

   EF1a

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-SpCas9-HF1

   Plasmid

   #126762

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_EF1aUBA3_IRES_Puro

   Plasmid

   #235660

   Purpose

   human UBA3 cloned into pLV-EF1a-IRES-Puro vector (Addgene: 85132)

   Depositor

   Lorenz Studer

   Article

   Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

   Insert

   UBA3 (UBA3 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-GRAB_NPY1.0

   Plasmid

   #208675

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. B-Sniper SpCas9

   Plasmid

   #207361

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAP03-sp44/p21

   Plasmid

   #120232

   Purpose

   Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Insert

   bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
   

   Use

   Synthetic Biology

   Mutation

   aac(3)IV (apramycin resistance gene)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. VP16-ER beta (long)

   Plasmid

   #11352

   Depositor

   Donald McDonnell

   Article

   Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

   Insert

   ER beta long (ESR2 Human)
   

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28-Strep-OPA1(253-960) (SB263)

    Plasmid

    #227612

    Purpose

    Inducible expression of Strep-tagged Opa1(253-960) in E.coli

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    OPA1 (OPA1 Human)
    

    Tags

    Twin-StrepTag

    Expression

    Bacterial

    Mutation

    contains aa 253-960 only

    Promoter

    T7

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-GRAB_NPY1.0

    Plasmid

    #208676

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP TEM4 FL

    Plasmid

    #58893

    Purpose

    Expresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.

    Depositor

    Channing Der

    Article

    Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

    Insert

    TEM4 (ARHGEF17 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L722F

    Promoter

    CMV

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28-Strep-OPA1(MGD) (SB264)

    Plasmid

    #227613

    Purpose

    Inducible expression of Strep-tagged Opa1(MGD) in E.coli

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    OPA1 (OPA1 Human)
    

    Tags

    Twin-StrepTag

    Expression

    Bacterial

    Mutation

    contains aa 253-580 (MGD) and aa 938-960

    Promoter

    T7

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP2440

    Plasmid

    #72250

    Purpose

    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Circular 200,100 (mPCSK9)

    Plasmid

    #170122

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Pcsk9 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6 and mouse U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV_EF1a-NAE1_IRES_Puro

    Plasmid

    #235659

    Purpose

    human NAE1 cloned into into lentiviral backbone (pLV-EF1a-IRES-Puro)

    Depositor

    Lorenz Studer

    Article

    Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

    Insert

    NAE1 (NAE1 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB_VIP1.0

    Plasmid

    #208681

    Purpose

    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL8 lenti-D10Acas9-3xflag-blast

    Plasmid

    #112134

    Purpose

    lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    D10Acas9-3xflag-blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A mutant in Cas9

    Promoter

    EF1a

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pH6-TEV-spTorA-GFP(C)

    Plasmid

    #169041

    Purpose

    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-GFP
    

    Tags

    6xHis, TEV cleavage site, and spTorA (signal pept…

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-GRAB_VIP1.0

    Plasmid

    #208682

    Purpose

    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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