We narrowed to 9,753 results for: try

1. pKK-TEV-mCardinal

   Plasmid

   #105798

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mCardinal

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKK-TEV-mCerulean

   Plasmid

   #105790

   Purpose

   Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mCerulean

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-mCardinal-TEV

   Plasmid

   #105781

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mCardinal-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-mCerulean-TEV

   Plasmid

   #105773

   Purpose

   Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mCerulean-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLuc-OptoJNKi

   Plasmid

   #89744

   Purpose

   Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-2B-Nkx2-5YRD Y-A

   Plasmid

   #98611

   Purpose

   Gateway entry vector for NKX2-5 YRD Y-A

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 YRD Y-A (Nkx2-5 Mouse)
   

   Use

   Entry vector

   Mutation

   Substitutions of 7 tyr into Ala residues.Y233A; Y…

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. KC1G3_HUMAN_D0

   Plasmid

   #79685

   Purpose

   This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KC1G3 (CSNK1G3 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. csd6_13-330_W158A/pET28b(+) N-tag

   Plasmid

   #71358

   Purpose

   csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

   Depositor

   SeWon Suh

   Article

   Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

   Insert

   HP0518 (HP0518 changed Tryptophan 158 to Alanine)
   

   Tags

   Histidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pFOSGE_COF4D03

   Plasmid

   #70969

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Taf12 (Taf12 Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pFOSGE_CUS1F03

    Plasmid

    #71071

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Su(var)205 (Su(var)205 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pDONR221-ARL4D-no stop

    Plasmid

    #67337

    Purpose

    hArl4D without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Gateway entry vector

    Expression

    Bacterial

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    None

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pDONR221-ARF4-no stop

    Plasmid

    #67311

    Purpose

    hArf4 without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Gateway entry vector

    Expression

    Bacterial

    Mutation

    V53A, L123P and M134I mutations were unintended P…

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pENTR1A DHFR.dn-cHSF1

    Plasmid

    #134726

    Purpose

    Gateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    DHFR.dn-cHSF1 (HSF1 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    DHFR

    Promoter

    None

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-MBP-TEV

    Plasmid

    #105771

    Purpose

    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    MBP-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p5E-Runx1+23

    Plasmid

    #69602

    Purpose

    Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

    Depositor

    Leonard Zon, Owen Tamplin

    Article

    Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

    Inserts

    Runx1 +23 intronic enhancer (Runx1 Mouse)
    Mouse beta-globin minimal promoter
    

    Use

    5' gateway entry vector

    Promoter

    C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKK-BiFC-Venus

    Plasmid

    #105804

    Purpose

    Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    VN173 (I152L); VC155

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)_Nav1.6WT-HA

    Plasmid

    #239762

    Purpose

    Expresses mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag_HsIRE1a_pBabePuro

    Plasmid

    #54337

    Purpose

    N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    The endogenous IRE1a signal sequence (aa1-18) was…

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only