We narrowed to 10,414 results for: 158

1. pTW199b2

   Plasmid

   #136928

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gIII
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR1265

   Plasmid

   #111094

   Purpose

   lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-enAsCas12f

   Plasmid

   #204637

   Purpose

   Expression of enAsCas12f in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   enAsCas12f
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D196K, N199K, G276R, N328G, D364R

   Promoter

   CMV

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDAC569

   Plasmid

   #195241

   Purpose

   Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT29

   Plasmid

   #138521

   Purpose

   phage cloning

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   phage genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMH062

   Plasmid

   #225537

   Purpose

   Expresses IS621 in insect cells

   Depositor

   Hiroshi Nishimasu

   Article

   Hiraizumi et al Nature. 2024 Jun;630(8018):994-1002. doi

   Insert

   IS621
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT423

   Plasmid

   #139382

   Purpose

   Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Constitutively expressed dCas9, LacI and TetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   P1

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMR-A-5

   Plasmid

   #138519

   Purpose

   P3c

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   T7-RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Reporter-2

   Plasmid

   #180219

   Purpose

   Surrogate reporter plasmid for the assay of targeted RNA editing

   Depositor

   Wensheng Wei

   Article

   Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

   Insert

   Surrogate reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTW211b5

    Plasmid

    #136927

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9(N)-NpuN
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    see manuscript

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-puro GFP-DFCP1

    Plasmid

    #38269

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHCMV-SS-antiMoonTagNb-TM

    Plasmid

    #198305

    Purpose

    Displays anti-MoonTag nanobody from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiMoonTagNb-TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. U6-AsCas12f-sgRNA-v2

    Plasmid

    #204639

    Purpose

    Expression of trancated AsCas12f sgRNA in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    trancated AsCas12f sgRNA (sgRNA-v2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUCIDT-AMP-Bv16SrRNA

    Plasmid

    #218518

    Purpose

    pUCIDT-AMP containing 16S rRNA gene of B. vulgatus for absolute concentration quantification

    Depositor

    Ami Bhatt

    Article

    Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

    Insert

    16S rRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid

    #195854

    Purpose

    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    6xHis-AviTag-ALFAtag-eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DL015:CMV::iPAK4

    Plasmid

    #177880

    Purpose

    ibox-PAK4cat (iPAK4) fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-10His-StayGold

    Plasmid

    #211362

    Purpose

    Mammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    StayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DL016:CMV::eGFP-iPAK4

    Plasmid

    #177881

    Purpose

    eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DL017:CMV::HaloTag-iPAK4

    Plasmid

    #177882

    Purpose

    HT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAWP78-PVCpnf_pvc13-CD4DARPin

    Plasmid

    #198294

    Purpose

    Produces PVCs retargeted with anti-CD4 DARPin D27.2

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-CD4DARPin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAWP78-PVCpnf_pvc13-FLAG

    Plasmid

    #198297

    Purpose

    Produces PVCs retargeted with FLAG tag

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-FLAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CA-0030 CMVTO-Renin-ECL

    Plasmid

    #231189

    Purpose

    Membrane-bound human renin

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    Renin-ECL (REN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBT138b-proB

    Plasmid

    #138515

    Purpose

    P3f

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBT100.164

    Plasmid

    #138522

    Purpose

    phage cloning

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(7.10)-inteinN
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAWP78-PVCpnf_pvc13-CD11bDARPin

    Plasmid

    #198295

    Purpose

    Produces PVCs retargeted with anti-CD11b DARPin F7

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-CD11bDARPin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p102_gRNA_Sa_mClover

    Plasmid

    #213778

    Purpose

    Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sa gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p103_gRNA_Sp_mClover

    Plasmid

    #213779

    Purpose

    Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sp gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMR-A-3

    Plasmid

    #138517

    Purpose

    P3a

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNTI819

    Plasmid

    #216628

    Purpose

    Cal-ID (V5) for mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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