We narrowed to 9,178 results for: mel

1. pLib6.4-Agam_557

   Plasmid

   #176653

   Purpose

   Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLib6.4B-Aalb_726

   Plasmid

   #176675

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLib6.4B-Aaeg_763

   Plasmid

   #176672

   Purpose

   Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLib6.4-Aaeg_972

   Plasmid

   #176660

   Purpose

   Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLib6.4-Cquin_801

   Plasmid

   #176657

   Purpose

   Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLib6.4B-Cquin_801

   Plasmid

   #176671

   Purpose

   Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLib6.4B-Cquin_728

   Plasmid

   #176670

   Purpose

   Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MMMMA-pHG165c

   Plasmid

   #170108

   Purpose

   Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.

   Depositor

   Liskin Swint-Kruse

   Article

   Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

   Insert

   MelR
   

   Expression

   Bacterial

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM1160c2

   Plasmid

   #159724

   Purpose

   Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Tags

   Gal4DBD and MPGIL-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM1674

    Plasmid

    #159726

    Purpose

    Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    mDHFR (Dhfr Mouse)
    

    Tags

    Gal4DBD and MPTLV-GGGGGG

    Expression

    Yeast

    Mutation

    remove start and stop codon of Dhfr

    Promoter

    ADH1

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM1479a3

    Plasmid

    #159725

    Purpose

    Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    mDHFR (Dhfr Mouse)
    

    Tags

    Gal4DBD and MYITV-GGGGGG

    Expression

    Yeast

    Mutation

    remove start and stop codon of Dhfr

    Promoter

    ADH1

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHCKan-yibDp-CBD-hGLY

    Plasmid

    #134596

    Purpose

    Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    human glycine acyltransferase 2 (GLYATL2 )
    

    Tags

    chitin binding domain

    Expression

    Bacterial

    Promoter

    yibD

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-T2-GpNLuc

    Plasmid

    #135949

    Purpose

    Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF1a-T2-OgNLuc

    Plasmid

    #135951

    Purpose

    Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSTAP-seq_fly-ssp3

    Plasmid

    #86382

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ssp3 enhancer
    

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSTAP-seq_fly-ham

    Plasmid

    #86383

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ham enhancer
    

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSTAP-seq_fly-sgl

    Plasmid

    #86384

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster sgl enhancer
    

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSTAP-seq_fly-ncm

    Plasmid

    #86385

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ncm enhancer
    

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSMART-Ala1

    Plasmid

    #65814

    Purpose

    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    NADPH Alanine Dehydrogenase (ald Synthetic)
    

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    Insulated waaHp from E. coli

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-pA-Hia5

    Plasmid

    #174372

    Purpose

    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein A - hia5
    

    Tags

    6xHis and Protein A

    Expression

    Bacterial

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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