We narrowed to 29,617 results for: ple

1. pCfB2803

   Plasmid

   #63646

   Purpose

   pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiU6-hNEUROD1 gRNA_1-MS2-Puro

   Plasmid

   #192676

   Purpose

   Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human NEUROD1 activating gRNA #1 (NEUROD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. RS17060_pMQ30_KO_Kan

   Plasmid

   #185396

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS17060 upstream flanking homology region
   RR42_RS17060 downstream flanking homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. RS17055_pMQ30_KO_Kan

   Plasmid

   #185398

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS17055 upstream flanking homology region
   RR42_RS17055 downstream flanking homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

   Plasmid

   #84258

   Purpose

   Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgTRE3G
   PYL1-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and mouse U6

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dMASS_1

   Plasmid

   #209764

   Purpose

   Expression of dMASS_1 in mammalian cells under CMV promoter

   Depositor

   Andre Berndt

   Article

   Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

   Insert

   dMASS_1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVH016

   Plasmid

   #80400

   Purpose

   No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).

   Depositor

   Richard Murray

   Article

   Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

   Inserts

   ffGFP-3xFLAG
   CusR (cusR )
   AraC (araC )
   

   Use

   Synthetic Biology

   Tags

   C-terminal 3xFLAG tag and C-terminal LZx domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPpT4- HTA1prom-TIR1 F74G -FLAG

   Plasmid

   #189728

   Purpose

   Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   HTA1

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB2797

   Plasmid

   #63639

   Purpose

   pCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLiP-G1

    Plasmid

    #188963

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA: atcggtcgcattgttttccactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB2804

    Plasmid

    #63644

    Purpose

    pCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCfB2875

    Plasmid

    #63640

    Purpose

    pCfB2875 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCherry-E2

    Plasmid

    #175242

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pgRNA_hMECP2_promoter_No4

    Plasmid

    #194899

    Purpose

    Lentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Insert

    sgRNA targeting human MECP2 promoter No.4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB2989

    Plasmid

    #63636

    Purpose

    pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEE495

    Plasmid

    #149291

    Purpose

    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CyPet-FKBP-E2

    Plasmid

    #175244

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

    Plasmid

    #192681

    Purpose

    Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB2802

    Plasmid

    #63655

    Purpose

    pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB2990

    Plasmid

    #63645

    Purpose

    pCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge