We narrowed to 13,746 results for: TIM;

1. pPD95.75-cYAP-1 S104A

   Plasmid

   #66858

   Purpose

   Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

   Insert

   YAP-1 (yap-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Serine104 is mutated to alanine

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. p300 KAT LIC 1M

   Plasmid

   #233587

   Purpose

   Bacterial expression of p300 KAT enzyme

   Depositor

   Scott Showalter

   Article

   Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

   Insert

   p300 KAT (EP300 Human)
   

   Use

   Tags

   6xHis MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28:LanIII

   Plasmid

   #225075

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIII)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIN1 (gpdA-cTerm-mGL-ptrA)

   Plasmid

   #188735

   Purpose

   Expresses mGreenlantern for C-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-mGreenlantern PtrA
   

   Use

   Filamentous fungal expression

   Tags

   mGreenlantern

   Expression

   Mutation

   Promoter

   gpdA

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIN2 (gpdA-nTerm-mGL-ptrA)

   Plasmid

   #188736

   Purpose

   Expresses mGreenlantern for N-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-mGreenlantern PtrA
   

   Use

   Filamentous fungal expression

   Tags

   mGreenlantern

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIN3 (gpdA-cTerm-Citrine2-ptrA)

   Plasmid

   #188737

   Purpose

   Expresses Citrine2 for C-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-Citrine2 PtrA
   

   Use

   Filamentous fungal expression

   Tags

   Citrine2

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIN4 (gpdA-nTerm-Citrine2-ptrA)

   Plasmid

   #188738

   Purpose

   Expresses Citrine2 for N-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-Citrine2 PtrA
   

   Use

   Filamentous fungal expression

   Tags

   Citrine2

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIN5 (gpdA-cTerm-mTurqoise2-ptrA)

   Plasmid

   #188739

   Purpose

   Expresses mTurqoise2 for C-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-mTurquoise PtrA
   

   Use

   Filamentous fungal expression

   Tags

   mTurquoise

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIN6 (gpdA-nTerm-mTurqoise2-ptrA)

   Plasmid

   #188740

   Purpose

   Expresses mTurqoise2 for N-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-mTurqoise2 PtrA
   

   Use

   Filamentous fungal expression

   Tags

   mGreenlantern

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUt-MONARCH 1.0_crEGFP

    Plasmid

    #224790

    Purpose

    LPUtopia matching RMCE donor plasmid with MONARCH 1.0 circuit with crRNA targeting EGFP. Use BlastR for positive and HSV-TK for negative selection

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crEGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-d2i

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUt-MONARCH 2.0_crEGFP

    Plasmid

    #224793

    Purpose

    LPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Inserts

    crEGFP
    Triplex stablier
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-d2i

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC-hU6-2O-crBFP_EF1a-BFP

    Plasmid

    #224783

    Purpose

    BFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC-hU6-2O-crEGFP_EF1a-BFP

    Plasmid

    #224784

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crEGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC-hU6-crBFP_EF1a-BFP

    Plasmid

    #224785

    Purpose

    BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC-hU6-CrEGFP_EF1a-BFP

    Plasmid

    #224786

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28:LanIV-Streptomyces

    Plasmid

    #226250

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIV-Streptomyces)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-SdrG-B2-ELP-HIS-ybbR

    Plasmid

    #169134

    Purpose

    E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    

    Use

    Tags

    3xELP linker, 6x Histag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-Fgß-FIVAR-ELP-HIS-ybbR

    Plasmid

    #169136

    Purpose

    E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    FIVAR (found in various architectures)
    

    Use

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-VHH-ddFLN4-HIS-ybbR

    Plasmid

    #169137

    Purpose

    E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    VHH
    

    Use

    Tags

    6x His tag, ddFLN4 tag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid

    #169133

    Purpose

    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    

    Use

    Tags

    3xELP linker, 6x Histag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge