We narrowed to 4,862 results for: Mela

1. pENTR-E2F1

   Plasmid

   #37141

   Depositor

   Robert Duronio

   Article

   Shibutani et al Dev Cell. 2008 Dec;15(6):890-900.

   Insert

   E2F1 (E2f1 Fly)
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

   Plasmid

   #149507

   Purpose

   Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   MFSD12 (MFSD12 Human)
   

   Use

   Transient expression

   Tags

   TEV-3XHA-3XFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized Sequence, p.LL256-257AA

   Promoter

   CMV

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTIGER_tdTomato::3xFLAG::dCrk

   Plasmid

   #131138

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   3XFLAG and tdTomato

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti6/V5-DEST-HOXA1

   Plasmid

   #31209

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   HOXA1 (HOXA1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

5. Pyr pUAST-HA

   Plasmid

   #69768

   Purpose

   Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

   Depositor

   Arno Muller

   Article

   Klingseisen et al Development. 2009 Jul;136(14):2393-402.

   Insert

   Pyramus (FGF8like-2) (pyr Fly)
   

   Use

   P element-based puast vector for gal4-regulated e…

   Tags

   HA-Tag

   Expression

   Insect

   Mutation

   241T amino acid insertion compared to reference s…

   Promoter

   hsp70 promoter

   Available Since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-2T-TRR-C421-Y2404F

   Plasmid

   #182846

   Purpose

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDF1-MCS2-EF1-Puro-RGS7

   Plasmid

   #113728

   Purpose

   Express RGS7 in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

   Insert

   RGS7 (RGS7 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. gRNAs[bTub+Sxl].1046B

   Plasmid

   #112692

   Purpose

   express two gRNA targeting bTub & Sxl under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KHBD00649

   Plasmid

   #39711

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG6172 (vnd Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pAc5.1B-EGFP-DmThor-V5His6

    Plasmid

    #79408

    Purpose

    Expresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor (Thor Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP and V5-His6

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

    Plasmid

    #113041

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Tag4A-MMP8 (P78S)

    Plasmid

    #29546

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    P78S

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pGL3-DICER-Prom Ebox double mut

    Plasmid

    #25854

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Promoter Ebox double mut (DICER1 gene ID 23405, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

    Plasmid

    #113040

    Purpose

    AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

    Plasmid

    #113042

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti6/V5-DEST-DEPDC1

    Plasmid

    #31206

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    DEPDC1 (DEPDC1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pGL3-DICER-Prom upstream Ebox Mut

    Plasmid

    #25852

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Promoter upstream Ebox Mut (DICER1 gene ID 23405, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-DICER-Prom downstream Ebox Mut

    Plasmid

    #25853

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    DICER-Prom downstream Ebox Mut (DICER1 gene ID 23405, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KHBD00517

    Plasmid

    #39618

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5893 (D Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

20. AcrIIC1X* (AcrIIC1X-mCherry chimera)

    Plasmid

    #128114

    Purpose

    Expresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9

    Depositor

    Dominik Niopek

    Article

    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

    Insert

    AcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge