We narrowed to 230,040 results for: addgene

1. pLPC-NMYC-mTRF1

   Plasmid

   #64162

   Purpose

   Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

   Depositor

   Titia de Lange

   Article

   Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

   Insert

   mTRF1 (Terf1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBabe puro Myr HA PIK3CB

   Plasmid

   #12527

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CB (PIK3CB Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Myristoylation. Constitutively active allele.

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. IFNGR2_pCSdest

   Plasmid

   #53823

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   IFNGR2 (IFNGR2 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pBABE MDER

   Plasmid

   #13494

   Depositor

   Stephen Tapscott

   Article

   Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

   Insert

   MyoD1-Estrogen Receptor Fusion Protein (Myod1 Mouse, Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   full length MyoD was fused to aa282-595 of Esr1. …

   Available Since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEG R2-iBlast-L3 #BGV204

   Plasmid

   #48982

   Purpose

   Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   blasticidin-S deaminase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. BPK2797

   Plasmid

   #72251

   Purpose

   Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, and T1337R

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCT-GAL93-SFPQ

   Plasmid

   #46320

   Depositor

   Steven Brown

   Article

   Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

   Insert

   SFPQ (SFPQ Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBEG R3-miRNA(ccdB)-L4 #BGV228

   Plasmid

   #48953

   Purpose

   To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Gateway cloning vector

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pBEG R2-iHygro-L3 #BGV206

   Plasmid

   #48985

   Purpose

   Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   hygromycin phosphotransferase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. F-CDK8L

    Plasmid

    #24762

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Conaway et al FEBS Lett. 2005 Feb 7. 579(4):904-8.

    Insert

    F-CDK8L (CDK19 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 14, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pEBG-PELP1

    Plasmid

    #65209

    Purpose

    Full length mammalian expression vector for hPELP1

    Depositor

    Ratna Vadlamudi

    Article

    Nair et al Cancer Res. 2010 Sep 15;70(18):7166-75.

    Insert

    PELP1 (PELP1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. PX458_AHR_2

    Plasmid

    #101077

    Purpose

    Encodes gRNA for 3' target of human AHR

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against AHR (AHR Human)
    

    Use

    CRISPR

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDR348 FANCF

    Plasmid

    #47510

    Purpose

    human gRNA expression vector targeting FANCF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-FANCF (FANCF Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

    Plasmid

    #99144

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

    Plasmid

    #52494

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE-puro-mIR-31 sponge

    Plasmid

    #22694

    Purpose

    Stable suppression of miR-31 function

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    miR-31 (MIR31 Human)
    

    Use

    Retroviral

    Tags

    *

    Expression

    Mammalian

    Mutation

    miR-31 sponge with 7x tandem binding sites to the…

    Available Since

    March 11, 2010

    Availability

    Academic Institutions and Nonprofits only

17. C2CD2_pCSdest

    Plasmid

    #53916

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    C2CD2 (C2CD2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1

    Plasmid

    #46676

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-133a-1 (MIR133A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. p3XFlag-Mettl4

    Plasmid

    #86665

    Purpose

    Expresses murine Mettl4 containing N-terminal 3XFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Mettl4 (Mettl4 Mouse)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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