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Showing: 41 - 80 of 1241 results

  11. pCS2+-FLAG3-drN-hsAgo2

    Plasmid
    #221724
    Purpose
    Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    drN-hsAgo2 (AGO2 Human)
    Use
    Fish expression
    Tags
    3xFLAG
    Expression
    Mutation
    Swap of N-domain for zebrafish Ago2 N-domain
    Promoter
    simian CMV IE94
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221724-plasmid-map-sequence-id-449653

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  20. pCIneo-lambdaN-HA-HsAGO2-P593GA623F_AG

    Plasmid
    #148743
    Purpose
    Mammalian Expression of HsAGO2-P593GA623F
    Depositor
    Elisa Izaurralde
    Insert
    HsAGO2-P593GA623F (AGO2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Three silent mutations and sequence differs in th…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148743-plasmid-map-sequence-id-291191

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  32. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Expression
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

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  34. pETM-60-HsAGO2-448-859-optim_K

    Plasmid
    #146729
    Purpose
    Bacterial Expression of HsAGO2_448-859-optim
    Depositor
    Elisa Izaurralde
    Insert
    HsAGO2_448-859-optim (AGO2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146729-plasmid-map-sequence-id-289175

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  38. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

    Plasmid
    #218513
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    deletion of the Plac_mrfp cassette
    Promoter
    PgyrA_ermBL-ermB'
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218513-plasmid-map-sequence-id-444102

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  40. BtARR1

    Plasmid
    #236262
    Purpose
    Bacterial expression of bovine arrestin-1 with inserted GGT codon after starting ATG
    Depositor
    Vsevolod Gurevich
    Insert
    arrestin-1 (SAG Bovine)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted GGT codon after starting ATG
    Promoter
    lac
    Available since
    May 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    236262-plasmid-map-sequence-id-472929

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Showing: 41 - 80 of 1241 results