We narrowed to 275 results for: petD
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Plasmid#223734PurposeExpression of recombinant protein for purificationDepositorInsertFUNDC1 soluble part (FUNDC1 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationaa 1-50 onlyAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE Y165A
Plasmid#139428PurposeGST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged tyrosine 165 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE P199A
Plasmid#139427PurposeGST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged proline 199 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE I137A
Plasmid#139319PurposeGST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged isoleucine 137 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(R37P)-RagC
Plasmid#99646PurposeBacterial expression of RagA(R37P)-RagCDepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(K84T))
Plasmid#99648PurposeBacterial expression of RagA-RagC(K84T)DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase
Plasmid#223747PurposeExpression of recombinant protein for purificationDepositorInsertLambda-PPase
Tags6xHis-TEVExpressionBacterialAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 Hepatitis C Virus NS3/NS4A
Plasmid#61696Purposeinducible dual expression of NS3 protease along with its cofactor NS4ADepositorInsertsNS4A
NS3
TagshisExpressionBacterialPromoterT7Available SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
[pSK122] pETDuet GST-p18(delta5)
Plasmid#136144Purposebacterial expression of GST-p18DepositorInsertGST-p18
TagsGSTExpressionBacterialAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
Tags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
Plasmid#85111Purposeexpression of recombinant human mature (delta19) ETFalpha wt without tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)
Plasmid#61939PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coliDepositorInsertsTags6x HISExpressionBacterialMutationConstruct ends at residue 156PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)
Plasmid#61938PurposeCo-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coliDepositorTags6x HISExpressionBacterialMutationConstruct ends at residue 156, residues 2-38 dele…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)
Plasmid#61943PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coliDepositorInsertsTags6x HISExpressionBacterialMutationResidues 2-39 deleted resulting in construct star…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-D282A
Plasmid#240407Purposeexpresses N-His tagged NocO D282A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, Asp…PromoterT7Available SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_6xHis-FKBP8(1-391aa)-thrombin-GST
Plasmid#227712PurposeExpression of recombinant protein for purificationDepositorInsertFKBP8(1-391aa) (FKBP8 Human)
ExpressionBacterialAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_6xHis-CCPG1(1-212aa)-thrombin-GST
Plasmid#227713PurposeExpression of recombinant protein for purificationDepositorInsertCCPG1(1-212aa) (CCPG1 Human)
ExpressionBacterialAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-H286A
Plasmid#240409Purposeexpresses N-His tagged NocO H286A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, His…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-H132A
Plasmid#240404Purposeexpresses N-His tagged NocO H132A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, His…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-E129A
Plasmid#240403Purposeexpresses N-His tagged NocO E129A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, Glu…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-Q128A
Plasmid#240402Purposeexpresses N-His tagged NocO Q128A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, Glu…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-E252A
Plasmid#240405Purposeexpresses N-His tagged NocO E252A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, Glu…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-E283A
Plasmid#240408Purposeexpresses N-His tagged NocO E283A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, Glu…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-H279A
Plasmid#240406Purposeexpresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, His…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-E94A
Plasmid#240401Purposeexpresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, Glu…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (WT)
Plasmid#223736PurposeExpression of recombinant protein for purificationDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (WT)
Plasmid#223737PurposeExpression of recombinant protein for purificationDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST
Plasmid#223745PurposeExpression of recombinant protein for purificationDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-LanCL2-H264A-C-Ter-His
Plasmid#154187PurposeTo express LanCL2-H264A in bacterial cellsDepositorInsertLanCL2
TagsHexahistidineExpressionBacterialMutationHis264->Ala264Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)
Plasmid#61941PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coliDepositorTags6x HISExpressionBacterialMutationConstruct ends at residue 117, stop codon introdu…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
213-petDuet1-GBD-LUMP--cdc42
Plasmid#65973PurposeDual expression of GBD-LUMP and cdc42DepositorInsertsTagsGBD (GTPase binding domain from ACK1; residues 44…ExpressionBacterialMutationconstitutively active Cdc42 (Q61L)PromoterT7Available SinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)
Plasmid#156463PurposeExpress Heme Oxygenase (Nostocaceae) and ReBphP-PCMDepositorInsertsHeme Oxygenase
ReBphP-PCM
TagsHis TagExpressionBacterialPromoterT7Available SinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)
Plasmid#182395PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusionsDepositorInsertsVNp-LZ-VenusN154
VNp-LZ-VenusC155
TagsVesicle Nucleating peptide (VNp) & Leucine Zi…ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A)
Plasmid#223751PurposeExpression of recombinant protein for purificationDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST-AUP1 379-410:Ube2G2
Plasmid#185335PurposeUsed for co-expression of the AUP1 G2BR with UBE2G2 for crystallographyDepositorAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-LanCL2-C321A/C367A-C-Ter-His
Plasmid#154188PurposeTo express LanCL2-C321A/C367A in bacterial cellsDepositorInsertLanCL2
TagsHexahistidineExpressionBacterialMutationCys321->Ala321; Cys367->Ala367Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS
Plasmid#61940PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coliDepositorTags6x HISExpressionBacterialMutationConstruct ends at residue 156, residues 97-100 (N…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_6xHis-TEV-mCherry-ATG13 IDR(230-517aa)
Plasmid#227711PurposeExpression of recombinant protein for purificationDepositorInsertmCherry-ATG13 IDR(230-517aa) (ATG13 Human)
ExpressionBacterialAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa)
Plasmid#223790PurposeExpression of recombinant protein for purificationDepositorInsertWIPI2d IDR (WIPI2 Human)
Tags6xHis-TEV-mCherryExpressionBacterialMutationaa 364-425 onlyAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)
Plasmid#223762PurposeExpression of recombinant protein for purificationDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)
Plasmid#223738PurposeExpression of recombinant protein for purificationDepositorInsertNIX (BNIP3L) soluble part (BNIP3L Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationW36A/L39A; deletion of LIRAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR)
Plasmid#223739PurposeExpression of recombinant protein for purificationDepositorInsertFUNDC1 soluble part, mutant (FUNDC1 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationY18A/L21A; deletion of LIRAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A; deltaLIR1)
Plasmid#223749PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-465, with W276A/I279A; deletion of…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)
Plasmid#223724PurposeExpression of recombinant protein for purificationDepositorInsertFIP200-CTR (RB1CC1 Human)
Tags6xHis-TEV-EGFPExpressionBacterialMutationaa 1429-1591 onlyAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
Plasmid#177849PurposeBacterial Expression of PNRDepositorInsertphotoreceptor cell-specific nuclear receptor (NR2E3 Human)
Tags6xHis and MBPExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A; deltaLIR3)
Plasmid#223775PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationI224A/L227A; deletion of LIR3Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I307A/V310A; deltaLIR4)
Plasmid#223776PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationI307A/V310A; deletion of LIR4Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A; deltaLIR2)
Plasmid#223783PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationY213A/I216A; deletion of LIR2Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (W276A/I279A; deltaLIR1)
Plasmid#223746PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationW276A/I279A; deletion of LIR1Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only