We narrowed to 378 results for: 52961

1. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Publication

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available since

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   3707 Articles

   

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These results may also be relevant.

1. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPRv2Cre

   Plasmid

   #82415

   Purpose

   Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

   Depositor

   David Feldser

   Publication

   Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

   Insert

   Cre Recombinase
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Mutation

   Mutated BsmBI cut site

   Promoter

   EFS (P2A)

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   7 Articles

   

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3. LcV2-Hygro

   Plasmid

   #91977

   Purpose

   LentiCRISPR v2 with Hygro cloned in place of Puro

   Depositor

   Joshua Mendell

   Publication

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   10 Articles

   

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4. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

   Plasmid

   #118154

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a core and U6

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   7 Articles

   

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5. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

   Plasmid

   #118156

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   1 Article

   

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6. LentiGuide-puro-NLS-mCherry

   Plasmid

   #185474

   Purpose

   Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Publication

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   4 Articles

   

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7. sgCtr- LentiCRISPRv2

   Plasmid

   #107402

   Purpose

   Lentiviral expression of Cas9 and a control gRNA

   Depositor

   William Kaelin

   Publication

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   control gRNA
   

   Use

   Lentiviral

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   7 Articles

   

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8. lentiCRISPR_DKO

   Plasmid

   #183192

   Purpose

   Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Publication

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6, mU6

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR-v2-FE-PuroR

   Plasmid

   #186746

   Purpose

   Modified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpin

   Depositor

   Richard Sherwood

   Publication

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   Cited by

   1 Article

   

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10. The Human Druggable CRISPR library

    Pooled library

    #200013

    Purpose

    Knockout library containing ~10,000 sgRNAs targeting 1,980 human druggable genes (5 sgRNAs per gene and 100 non-targeting control sgRNAs).

    Depositor

    Lianxin Liu

    Publication

    Zheng et al Adv Sci (Weinh). 2023 Aug;10(22):e220679

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

11. Human SLC KO Library

    Pooled library

    #132552

    Purpose

    Knockout library targeting human SLC proteins.

    Depositor

    Giulio Superti-Furga

    Publication

    Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    Cited by

    3 Articles

12. Human ubiquitination-related proteins CRISPR KO library

    Pooled library

    #174592

    Purpose

    Targets human ubiquitation-related proteins and deubiquitinating enzymes.

    Depositor

    Juan Bonifacino

    Publication

    Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    Cited by

    1 Article

13. pTRE_GFP-GluR1

    Plasmid

    #34857

    Depositor

    Mark Mayford

    Publication

    Matsuo et al Science. 2008 Feb 22;319(5866):1104-7.

    Insert

    GluR1 (Gria1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    TRE

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    Cited by

    1 Article

    

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14. LentiGuide-puro-NLS-GFP

    Plasmid

    #185473

    Purpose

    Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Publication

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available since

    July 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    Cited by

    5 Articles

    

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15. lenti-SpCas9 puro

    Plasmid

    #104994

    Purpose

    This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

    Depositor

    Brett Stringer

    Publication

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    Cited by

    1 Article

    

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16. lenti SYN-dCas9-KRAB-MeCP2

    Plasmid

    #155365

    Purpose

    Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Publication

    Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    3x FLAG and 7x His

    Expression

    Mammalian

    Promoter

    SYN

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    Cited by

    3 Articles

    

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17. LentiGuide-TagRFP-2A-BSD

    Plasmid

    #167930

    Purpose

    Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Publication

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    Cited by

    1 Article

    

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18. lenti-SpCas9 blast

    Plasmid

    #104997

    Purpose

    This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

    Depositor

    Brett Stringer

    Publication

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    Cited by

    6 Articles

    

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19. LentiGuide-TagBFP-2A-BSD

    Plasmid

    #167929

    Purpose

    Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Publication

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    Cited by

    1 Article

    

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20. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid

    #196727

    Purpose

    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    TRE

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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