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CROPSeq-BFP
Plasmid#196718PurposeCROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFPDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-BFP
Plasmid#120577PurposeLentiviral plasmid to co-express a guide RNA and EBFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
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BFP-KASH2
Plasmid#187019PurposeInducible disruption of LINC ComplexDepositorInsertKASH2
UseTagstagBFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
BFP-KASH2ext
Plasmid#187020PurposeControl for inducible disruption of LINC ComplexDepositorInsertKASH2ext
UseTagstagBFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-BFP
Plasmid#164273PurposeEmpty sgRNA expression vector with co-expression of BFP reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EFSAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSADdeltaG-BFP
Plasmid#32639DepositorInsertBFP
UseRabies virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
BFP-RanQ69L
Plasmid#163438PurposeMammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredationDepositorInsertRanQ69L (RAN Human)
UseTagstag-BFPExpressionMammalianMutationQ69LPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVII-BFP
Plasmid#162755PurposeRetroviral (MSCV) expression of mTagBFP2 fluorescent proteinDepositorInsertmTagBFP2
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-BFP
Plasmid#106282PurposeA version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.DepositorInsertCMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-BFP
Plasmid#155355PurposeLentivector to express CRY2olig-BFP controlDepositorInsertcry2olig-BFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
InterTag-BFP
Plasmid#186901PurposeCell-free expression of transmembrane protein PDGFR-tagBFP-sfCherry11-SpyTagDepositorInsertSpyTag003-sfCherry11-tagBFP-PDGFR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
sTag-BFP
Plasmid#186903PurposeCell-free expression of soluble protein tagBFP-sfCherry11-SpyTagDepositorInsertSpyTag003-sfCherry11-tagBFP
UseSynthetic BiologyTagstagBFPExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-BFP
Plasmid#179651PurposeBFP co-transfection markerDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pk335-BFP
Plasmid#227505PurposeBackbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistenceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
1BR9-BFP
Plasmid#202054PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged tagBFP.DepositorInsertBFP
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN BFP
Plasmid#188644PurposeThis is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genesDepositorTypeEmpty backboneUseRetroviralTagsBFPExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)
Plasmid#211767PurposedCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selectionDepositorInsertdCas9, Spy, Snoop, aGCN4, AviTags
UseTags3xHAExpressionMammalianMutationdCas9 D10A and H840APromoterpGK and pEF1aAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GalToxBFP
Plasmid#68073Purposemammalian expression of GC localized oxBFPDepositorInsertGalT signal anchor transmembrane domain
UseTagsmoxBFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only
