-
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianMutationPromoterdSV40 PromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE
Plasmid#60906PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pHR-Tre3G-47xCAG-12xMS2
Plasmid#99148PurposeEncodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vectorDepositorInsert47xCAG 12xMS2 hairpins
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCGN-MEF2B
Plasmid#32959PurposeFor mammalian expression of human MEF2B with an HA tagDepositorInsertMEF2B (MEF2B Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1
Plasmid#27156PurposeFor mammalian expression of human Elk-1 with an HA tagDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR-tdMCP-YFP
Plasmid#99151PurposeExpresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vectorDepositorInsertDimeric MS2-coat binding protein
UseLentiviralTagseYFPExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-Tre3G-29xGGGGCC-12xMS2
Plasmid#99149PurposeEncodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vectorDepositorInsert29xGGGGCC 12xMS2 hairpins
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-CTG-47
Plasmid#99150Purpose47xCAG repeats in pBluescript vectorDepositorInsert47xCTG
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19-FcGamma CAR
Plasmid#113014Purposefor production of lentivirus encoding the CD19 CAR-PfcgammaDepositorInsertCD19 CAR-P intracellular GFP (CD19 Synthetic, Human, Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_Ngn2‐2A‐Ngn1
Plasmid#61471Purpose3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn2_UBC_Puro
Plasmid#61474Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection markerDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro
Plasmid#60909PurposeA minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imagingDepositorInsertK560-E236A
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
XBP-1 1: pFLAG.XBP1u.CMV2
Plasmid#21832DepositorInsertXBP-1 unprocessed (Xbp1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-155-5p
Plasmid#103265PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-155-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-155-5p target (MIR155 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta
Plasmid#89347Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequencesDepositorInsertJurkat TCRalpha/beta alleles and CD3e and CD3z
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-K560-24xGCN4_v4
Plasmid#72229PurposeA fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide arrayDepositorInsertK560 kinesin
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_hEF1a_rtTA3
Plasmid#61472Purpose3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)DepositorInsertrtTA3
UseLentiviralTagsExpressionMutationPromoterhEF1aAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only