We narrowed to 414 results for: SAM activators

1. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124539

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55A, d11-41

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124540

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41, d11-41

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124538

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFD3-MS2tail-SAM

   Plasmid

   #78905

   Purpose

   Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Aug. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

   Plasmid

   #112925

   Purpose

   Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6 promoter, gRNA-SAM expression cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-SAM-hygro

   Plasmid

   #138955

   Purpose

   Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

   Depositor

   Nadya Dimitrova

   Article

   Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 AND EF1a

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

   Plasmid

   #160570

   Purpose

   A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNA:scaffold tetraloop MS2 aptamer SAM
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ESAM-bio-His

   Plasmid

   #51751

   Purpose

   Expresses full-length Endothelial cell-selective adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ESAM (ESAM Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

   Plasmid

   #112927

   Purpose

   CRISPR-a reporter assay lentiviral vector (gTetO)

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112926

    Purpose

    CRISPR-a reporter assay lentiviral vector (empty gRNA control)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(empty), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-SAM-Puro

    Plasmid

    #198713

    Purpose

    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a and U6

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-SAM-Cre

    Plasmid

    #198714

    Purpose

    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    Pgk and U6

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiSAMPHv2

    Plasmid

    #167934

    Purpose

    All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiSAM v2 (Puro)

    Plasmid

    #92062

    Purpose

    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (sgRNA) and EF1a (dCas9-VP64)

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

    Plasmid

    #226590

    Purpose

    Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.

    Depositor

    D. James Surmeier

    Insert

    PSAML-141F,Y115F-GlyR
    

    Use

    AAV

    Tags

    P2A-Fusion Red

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

    Plasmid

    #32474

    Purpose

    Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAMQ79G,Q139G-5HT3 HC
    

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiSAMv2 ZFAND3-guide4

    Plasmid

    #125516

    Purpose

    CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiSAMv2 ZFAND3-guide2

    Plasmid

    #125514

    Purpose

    CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiSAMv2 ZFAND3-guide1

    Plasmid

    #125513

    Purpose

    CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiSAMv2 ZFAND3-guide3

    Plasmid

    #125515

    Purpose

    CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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