Addgene

We narrowed to 44,043 results for: STI;

Showing: 21 - 40 of 44043 results

Results per page:

Sort results by:

pStI6

Plasmid

#8616

Depositor

Robert Hartley

Article

Krajcikova et al FEBS Lett 2004 Jan 16;557(1-3):164-8.

Insert

Ribonuclease inhibitor StI

Use

Tags

Expression

Bacterial

Mutation

S2G point mutation; 19 silent mutations improve p…

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

Plasmid

#187959

Purpose

Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Inserts

Blasticidin resistance
FKBP12_F36V

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNestin-EGFP

Plasmid

#38777

Depositor

Wei Cui

Article

Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

Insert

nestin 2nd intron enhancer with TK mini promoter (NES Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a MISTIC

Plasmid

#85999

Purpose

bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

N/A

Use

Tags

HIS6 and MISTIC

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-OptoSTIM1

Plasmid

#70159

Purpose

Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Article

Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

Insert

OptoSTIM1 (STIM1 Mustard Weed, Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv2-STING_gRNA3

Plasmid

#127640

Purpose

Knock-out of human STING

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-monSTIM1

Plasmid

#161795

Purpose

Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

MonSTIM1 (STIM1 Mustard Weed, Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Ostir-neo

Plasmid

#161973

Purpose

Expresses Ostir and G418 resistance gene in mammalian cells

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Ostir-3xHA-p2a-neoR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-M1 Spastin

Plasmid

#134461

Purpose

M1 Spastin expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Met to Ala change at amino acid 87

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZLblasti-HA-ERK5

Plasmid

#53175

Purpose

Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pOsTIR1w/oGFP

Plasmid

#102883

Purpose

Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

OsTIR1

Use

Tags

3x myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZLblasti-CTR1

Plasmid

#53157

Purpose

Expresses human CTR1 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
STIMATE-mCherry

Plasmid

#70676

Purpose

Expresses STIMATE or TMEM110 in mammalian cells

Depositor

Yubin Zhou

Article

Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

Insert

STIMATE (TMEM110 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
mApple-M1 SpastinE442Q

Plasmid

#134464

Purpose

M1 SpastinE442Q expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Glu to Gln at amino acid 442

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pOpen-PstIR

Plasmid

#165526

Purpose

Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

Depositor

FreeGenes Project, Drew Endy, Jennifer Molloy

Insert

PstI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PB-Ostir-puro

Plasmid

#161972

Purpose

Expresses Ostir and puromycin resistance gene in mammalian cells

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Ostir-3xHA-p2a-puroR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-M1 SpastinK388R

Plasmid

#134463

Purpose

M1 SpastinK388R expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Lys to Arg at amino acid 388

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNCX2-STIM1

Plasmid

#89817

Purpose

Express HUMAN STIM1

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

NO TAG

Expression

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mCerulean3-Plastin-C-10

Plasmid

#55438

Purpose

Localization: Fimbrin/Cytoskeleton, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Plastin (PLS1 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge