-
Plasmid#31384DepositorInserthPDI1 (P4HB Human)
UseTagsFLAG M1ExpressionMammalianMutationC400SPromoterAvailable sinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1
Plasmid#176838PurposeTo induce human PDX1 expression by recruiting SAM complex to PDX1 promoterDepositorInsertsgPDX1
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-coHPDL H258A 3xFlag-V5
Plasmid#174130PurposeExpresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTags3xFLAG-V5ExpressionMammalianMutationCodon optimized, H258APromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-coHPDL H163A H258A 3xFLAG-V5
Plasmid#174131PurposeExpresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTags3xFLAG-V5ExpressionMammalianMutationCodon optimized, H163A, H258APromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2
Plasmid#204852PurposeAAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouseDepositorInsertminiSdd6-nSaCas9KKH(D10A)-UGI
UseAAV and CRISPRTagsExpressionMutationD10A, E782K, N968K, R1015H in SaCas9PromoterEFS, miniU6Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 G224A-mGFP
Plasmid#180819PurposeExpression of human PD-1 G224A fused to mEGFPDepositorInsertPD-1 (PDCD1 Human)
UseLentiviralTagsmEGFPExpressionMutationG224APromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SP-GFP-CD274-NU
Plasmid#121139PurposeMammalian expression of GFP tagged CD274DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1-SNAP
Plasmid#223576PurposeLentiviral expression of human PD-1 with SNAP tag in mammalian cellsDepositorInsertPD-1 (PDCD1 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-PD-1
Plasmid#223572PurposeLentiviral expression of human PD-1 with SNAP tag in mammalian cellsDepositorInsertPD-1 (PDCD1 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-PD-1 I126A
Plasmid#223573PurposeLentiviral expression of human PD-1 (I126A) with SNAP tag in mammalian cellsDepositorInsertPD-1 (PDCD1 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationI126APromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-PD-1
Plasmid#223569PurposeProtein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cellsDepositorInsertPD-1 (PDCD1 Human)
UseTagsTwin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 (BTLA ITIM)-mGFP
Plasmid#180817PurposeExpression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFPDepositorInsertPD-1 (BTLA ITIM) (PDCD1 Human)
UseLentiviralTagsmEGFPExpressionMutationreplaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-4x
Plasmid#124678PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-6-C, 16-C
Plasmid#124681PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-1x
Plasmid#124679PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-Ce67
Plasmid#124680PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a(His10-PD-1cyto)
Plasmid#177896PurposeE coli expression of His10 tag fused to human PD-1 Intracellular DomainDepositorInsertPD-1 (aa 194-288 only) (PDCD1 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-12-C, 21-G
Plasmid#124683PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-1-A, 5-A
Plasmid#124682PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 (Y223F, Y248F)-mGFP
Plasmid#180790PurposeExpression of human PD-1 (Y223F, Y248F) fused to mEGFPDepositorInsertPD-1 (Y223F, Y248F) (PDCD1 Human)
UseLentiviralTagsmEGFPExpressionMutationPD-1 (Y223F, Y248F)PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only