We narrowed to 226 results for: lbcas12a

1. pYPQ230-D156R (ttLbCas12a)

   Plasmid

   #195366

   Purpose

   A temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12a

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   LbCas12a-D156R (ttLbCas12a)
   

   Use

   CRISPR; Gateway compatible lbcas12a-d156r (ttlbca…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMBP-LbCas12a D832A

   Plasmid

   #113517

   Purpose

   Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   D832A

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMBP-LbCas12a H759A

   Plasmid

   #113518

   Purpose

   Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   H759A

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

   Plasmid

   #192350

   Purpose

   L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   AATG_dttLbCas12a-i_TTCG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R; E925Q

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LbCas12a-DR-GC-swap-cr in pBCSK+

   Plasmid

   #126643

   Purpose

   Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a Full Length Direct Repeat GC Swap crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCJH031_proD_LbCas12a-gRNA-GFP_CAM

   Plasmid

   #183075

   Purpose

   Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a guide RNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS

   Plasmid

   #244836

   Purpose

   Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   2xSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEM4_CL7_Bac_2NLS-WT (LbCas12a)-4NLS

   Plasmid

   #244839

   Purpose

   Bacterial expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS

   Plasmid

   #244843

   Purpose

   Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus and 4xSV40 NLS at C-temrinus

   Depositor

   Jennifer Doudna

   Article

   Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

   Insert

   Lbm6-Cas12a
   

   Use

   CRISPR

   Tags

   10xHis-MBP and 4xSV40 NLS

   Expression

   Bacterial

   Mutation

   D535G/S551F/D665N

   Promoter

   T7

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-LbCas12a-8xNLS

    Plasmid

    #209020

    Purpose

    Lentiviral vector expressing human codon-optimized LbCas12a with 8xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    LbCas12a-8xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUPD2 LbCas12a (GB1439)

    Plasmid

    #160573

    Purpose

    Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    LbCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

    Plasmid

    #160611

    Purpose

    Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:LbCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-LbCas12a-Plus-mCherry

    Plasmid

    #191230

    Purpose

    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    LbCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R, R883K, R887A

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

    Plasmid

    #160575

    Purpose

    LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtU6-26:LbCas12a_DR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEM2_Euk_2NLS-RVQ (LbCas12a)-2NLS

    Plasmid

    #244837

    Purpose

    Mammalian expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR

    Tags

    2xSV40 NLS

    Expression

    Mammalian

    Mutation

    G146R/R182V/E795Q

    Promoter

    T7

    Available since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

    Plasmid

    #244844

    Purpose

    Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    iCas12a
    

    Use

    CRISPR

    Tags

    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

    Expression

    Bacterial

    Mutation

    F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…

    Promoter

    T7

    Available since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEM5_CL7_Bac_2NLS-Lbm6 (LbCas12a)-4NLS

    Plasmid

    #244840

    Purpose

    Bacterial expression of Lbm6 LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    Lbm6-Cas12a
    

    Use

    CRISPR

    Tags

    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

    Expression

    Bacterial

    Mutation

    D535G/S551F/D665N

    Promoter

    T7

    Available since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEM6_CL7_Bac_2NLS-RVQ (LbCas12a)-2NLS

    Plasmid

    #244841

    Purpose

    Bacterial expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    LbCas12a-RVQ
    

    Use

    CRISPR

    Tags

    10xHis-CL7-2xSV40 NLS and 2xSV40 NLS

    Expression

    Bacterial

    Mutation

    G146R/R182V/E795Q

    Promoter

    T7

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SM468_EF1A-dHyperLbCas12a-T2A-EGFP

    Plasmid

    #244220

    Purpose

    Mammalian expression of dHyperLbCas12a with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SM469_EF1A-dHyperLbCas12a-T2A-BlastR

    Plasmid

    #244221

    Purpose

    Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge