We narrowed to 7,664 results for: puc

1. pUC118

   Plasmid

   #50009

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Vieira et al Methods Enzymol. 1987;153:3-11.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC_sgRNA

   Plasmid

   #68710

   Purpose

   Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC21

   Plasmid

   #49787

   Purpose

   E. coli cloning vector (AmpR, high copy, blue/white selection, M13 IR)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC8-SZ

   Plasmid

   #17330

   Depositor

   Takeshi Sano

   Article

   Argarana et al Nucleic Acids Res. 1986 Feb 25. 14(4):18

   Insert

   streptavidin
   

   Use

   For maintenance

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pUC13

   Plasmid

   #50003

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCX

   Plasmid

   #60681

   Purpose

   E. coli expression plasmid, useful in conjunction with pUCXKT for positive selection cloning and expression

   Depositor

   David Ackerley

   Article

   Prosser et al Biotechnol Lett. 2014 Sep 26.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCXKT

   Plasmid

   #60682

   Purpose

   positive selection cloning and expression vector

   Depositor

   David Ackerley

   Article

   Prosser et al Biotechnol Lett. 2014 Sep 26.

   Type

   Empty backbone

   Use

   Positive selection e. coli expression vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SRAcode_in_pUC57_v3

   Plasmid

   #49822

   Purpose

   for transcribing SRA nucl 419-1356 of NM_001035235 with T7

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator RNA coding (SRA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   KpnI site at 5' start of RNA coding seq

   Promoter

   T7

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC6

   Plasmid

   #49793

   Purpose

   E. coli cloning vector

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1982 Oct;19(3):259-68.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. PUC9B71A

    Plasmid

    #44602

    Depositor

    Robert Oshima

    Article

    Singer et al J Biol Chem. 1986 Jan 15;261(2):538-47.

    Insert

    endoB (Krt18 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC12

    Plasmid

    #50002

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SP_gRNA_pUC19_N_FancF_Left

    Plasmid

    #79892

    Purpose

    Human gRNA expression vector targeting FANCF (site 1 left)

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Insert

    FancF_Site_1_Left
    

    Use

    CRISPR

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19_TTE1564

    Plasmid

    #61000

    Purpose

    T7-transcription template for Tte mRNA used for in vitro translation

    Depositor

    Nils Walter

    Article

    Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

    Insert

    TTE1564 transcript
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19_BAS1509

    Plasmid

    #82354

    Purpose

    T7 in vitro transcription template for Bas mRNA

    Depositor

    Nils Walter

    Article

    Chatterjee et al Proc Natl Acad Sci U S A. 2021 Apr 20;11

    Insert

    BAS1509 hypothetical protein
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19_T790M_28bp

    Plasmid

    #114721

    Purpose

    28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    c.2369C>T (Substitution, position 2369, C➞T); …

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19_4xT790_28bp

    Plasmid

    #114720

    Purpose

    4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19_T790_28bp

    Plasmid

    #114722

    Purpose

    28 bp fragment of the WT EGFR allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19_4xT790M_28bp

    Plasmid

    #114719

    Purpose

    4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    c.2369C>T (Substitution, position 2369, C➞T); …

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC:FCP:ShBle:FCP:EGFP

    Plasmid

    #85987

    Purpose

    Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

    Depositor

    Thomas Mock

    Article

    Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

    Inserts

    ShBle
    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    FCP

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19_extendedTTE1564

    Plasmid

    #60999

    Purpose

    T7-transcription template for Tte mRNA used in SiM-KARTS

    Depositor

    Nils Walter

    Article

    Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

    Insert

    TTE1564 transcript
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge