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Showing: 21 - 40 of 4933 results

  1. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Use
    Tags
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  3. PtPuc3_diaCas9_sgRNA

    Plasmid
    #109219
    Purpose
    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum
    Depositor
    Per Winge
    Article
    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    LHCF1 terminator, LHCF2 promoter, U6 3' regi…
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    109219-plasmid-map-sequence-id-260727

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  4. pUC19/N-Vc

    Plasmid
    #183157
    Purpose
    Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.
    Depositor
    Qinlong Zhu, Yao-Guang Liu
    Article
    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183157-plasmid-map-sequence-id-360347

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  13. pUC19/Vc-C

    Plasmid
    #183158
    Purpose
    Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.
    Depositor
    Qinlong Zhu, Yao-Guang Liu
    Article
    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183158-plasmid-map-sequence-id-360348

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  14. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68710-plasmid-map-sequence-id-123992

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  15. pUC21

    Plasmid
    #49787
    Purpose
    E. coli cloning vector (AmpR, high copy, blue/white selection, M13 IR)
    Depositor
    Joachim Messing
    Article
    Vieira et al Gene. 1991 Apr;100:189-94.
    Type
    Empty backbone
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    49787-plasmid-map-sequence-id-139618

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  16. pUC19_GZG

    Plasmid
    #117226
    Purpose
    shble flanked by an endogenous GAPDH promoter and terminator system
    Depositor
    Jackie Collier
    Insert
    shble
    Use
    Thraustochytrid expression
    Tags
    Expression
    Mutation
    Promoter
    GAPDH
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    117226-plasmid-map-sequence-id-224760

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  17. pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS

    Plasmid
    #163755
    Purpose
    piggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytes
    Depositor
    Kurt Pfannkuche
    Article
    Hamad et al Methods Mol Biol. 2021 Oct 19. doi: 10.1
    Insert
    mCherry
    Use
    Piccybac transposon
    Tags
    puromycin-N-acetyltransferase
    Expression
    Mammalian
    Mutation
    Promoter
    human NCX1
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163755-plasmid-map-sequence-id-326668

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Showing: 21 - 40 of 4933 results