We narrowed to 146 results for: single guide rna sgrna

1. pSuperCRISPR-sgRNA-RL69-TATT

   Plasmid

   #215849

   Purpose

   This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-RL69 with TATT stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSuperCRISPR-sgRNA-EGFP12-WT(TTTT)

   Plasmid

   #215856

   Purpose

   This plasmid encodes sgRNA that target EGFP with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-EGFP12 with TTTT stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSuperCRISPR-sgRNA-RL69-TTTC

   Plasmid

   #215855

   Purpose

   This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-RL69 with TTTC stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSuperCRISPR-sgRNA-RL69-TCTT

   Plasmid

   #215853

   Purpose

   This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-RL69 with TCTT stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSuperCRISPR-sgRNA-RL69-TTCT

   Plasmid

   #215854

   Purpose

   This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-RL69 with TTCT stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L4440_BioBrick-SCR-sgRNA-A

   Plasmid

   #177811

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L4440_BioBrick-hsf-1-sgRNA-A

   Plasmid

   #177786

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hsf-1 (hsf-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. L4440_BioBrick-hif-1-sgRNA-A

   Plasmid

   #177784

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L4440_BioBrick-hsf-1-sgRNA-B

    Plasmid

    #177787

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hsf-1 (hsf-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. L4440_BioBrick-hif-1-sgRNA-B

    Plasmid

    #177785

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hif-1 (hif-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDT-sgRNA-XMAS-2x

    Plasmid

    #164414

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNTI661 pRPR1(TetO)-sgRNA

    Plasmid

    #139475

    Purpose

    Vector for tet-regulated sgRNA expression in yeast

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Insert

    single guide RNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pRPR1(TetO)

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #159281

    Purpose

    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    AsCpf1-HA-2A-GFP
    

    Use

    Multicistronic vector with both caggs promoter-dr…

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiGuideFB-Puro-A

    Plasmid

    #192506

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCJH009_proD_Cas12c-sgRNA-GFP_CAM

    Plasmid

    #183070

    Purpose

    Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA targeting GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid

    #159283

    Purpose

    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Inserts

    Human INS sgRNA
    Human INS guide RNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cas12c-sgRNA_BbsI_CAM

    Plasmid

    #183221

    Purpose

    Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge