Addgene

We narrowed to 2,414 results for: t aav

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFP

Plasmid

#80491

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

CAG-GFP

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFPluc2

Plasmid

#80493

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

GFPluc2

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-mTubb3

Plasmid

#87116

Purpose

AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-nEFCas9

Plasmid

#87115

Purpose

SpCas9 expression AAV backbone plasmid under control of nEF promoter

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

nEF-Cas9

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-DeltaFosB

Plasmid

#68545

Purpose

AAV expression of DeltaFosB

Depositor

Eric Nestler

Article

Zachariou et al Nat Neurosci. 2006 Feb;9(2):205-11. Epub

Insert

DeltaFosB-IRES-GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

truncated splice variant of FosB

Promoter

CMV

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only
AAV-CREB

Plasmid

#68550

Purpose

AAV expression of CREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

CREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-mCREB

Plasmid

#68551

Purpose

AAV expression of mCREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

mCREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

S133A

Promoter

CMV

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
paavCAG-Jx

Plasmid

#51905

Purpose

Cre-dependent On/Off aav vector

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1 T2 CRIPR in pX330

Plasmid

#72833

Purpose

CRISPR/Cas to target the AAVS1 locus in human cells

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Cas9 and gRNA for targeting the AAVS1 locus in human cells

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-CAG-DEST

Plasmid

#80489

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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AAV GFP

Plasmid

#49055

Purpose

adeno-associated virus (AAV) encoding a green fluorescent protein

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

Plasmid

#35500

Purpose

AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

Plasmid

#35499

Purpose

AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E122T and E162T

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-HITI

Plasmid

#87117

Purpose

AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-tLuc-mCherry

Plasmid

#107553

Purpose

tLuc-mCherry in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-rMERTK-HITI

Plasmid

#87119

Purpose

Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-luc

Plasmid

#87118

Purpose

AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA

Use

AAV, CRISPR, and Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PdTK-CAG-mCh-[uBglII]

Plasmid

#107280

Purpose

puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )

Use

Tags

Expression

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-EC12WTC9AAvi

Plasmid

#47925

Purpose

Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

Depositor

Tanja Kortemme

Article

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Use

Tags

6xHis, Avi Tag, and TEV Cleavage

Expression

Bacterial

Mutation

changed Cysteine 9 to Alanine

Promoter

T7

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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