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Plasmid#67964PurposeMobilizible plasmid with EGFP. OriT from the F plasmid.DepositorInsertOriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
sg_shuttle_RFP657
Plasmid#134968PurposeLentiviral vector for sgRNA expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6-sgRNA; EFS-RFP657Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
ShuttleNSE
Plasmid#50958PurposeA backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoterDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterNSEAvailable sinceFeb. 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
U6 pShuttle
Plasmid#13428DepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEZYshuttle
Plasmid#18703PurposepShuttle-CMV converted Gateway Destination vector for AdEasy adenoviral vector systemDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTargeting_Mettl3C_FKBP-V
Plasmid#165421PurposeTemplate for knockin FBKP-V into Mettl3 C-terminusDepositorInsertMettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)
UseMouse TargetingTagsFKBP-VExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_U6_sgRNA_Mettl3C
Plasmid#165420PurposesgRNA construct for targeting Mettl3 C-terminusDepositorInsertPspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
ShuttleMCP
Plasmid#50959PurposeA backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoterDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoteruses portion of the mouse cofilin gene as a mouse…Available sinceFeb. 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pShuttle.Cre
Plasmid#192930PurposeGateway compatible vector with BsaI cloning site and Cre expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorTagsExpressionMutationPromoterCMVAvailable sinceDec. 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pShuttle.CC9
Plasmid#192931PurposeGateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorTagsExpressionMutationPromoterCMVAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-U6-TTLL11
Plasmid#140582PurposeExpresses a gRNA and mCherry in mammalian cellsDepositorInsertgRNA TTLL11
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV
Plasmid#16403PurposeShuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 METTL3
Plasmid#200731PurposeBacterial expression of GST-Tagged codon optimized human METTL3DepositorInsertMETTL3 (METTL3 Human)
UseTagsGST from backbone plasmidExpressionBacterialMutationCodon optimized for expression in E. coliPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-METTL14-WT
Plasmid#141112PurposeOverexpression of WT METTL14 in mammalian cells.DepositorInsertHMETTL14 (METTL14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-U6-sgMettl3
Plasmid#196263PurposeExpresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoterDepositorInsertMettl3 sgRNA (Mettl3 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1hWT
Plasmid#35027DepositorInsertFEN1 (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-Cre-HA
Plasmid#16583DepositorInsertcre recombinase (cre P1 bacteriophage)
UseAdenoviralTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
CAG pShuttle
Plasmid#13427DepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
5TEY (METTL3)
Plasmid#101892PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertMETTL3 (METTL3 Human)
UseBaculovirus expressionTags6xHis, TEVExpressionMutationPromoterpolyhedrinAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only