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We narrowed to 11,308 results for: ENA

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Showing: 951 - 1000 of 11308 results

  1. pLKO_NWS.AU1.V5_NGFR

    Plasmid
    #158237
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.AU1.V5
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158237-plasmid-map-sequence-id-327677

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  2. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

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  6. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  7. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid
    #124257
    Purpose
    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii
    Insert
    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    Expression
    Mammalian
    Mutation
    Arginine 132 is mutated to Histidine. This mutati…
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124257-plasmid-map-sequence-id-252091

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  8. pGLU_111

    Plasmid
    #225792
    Purpose
    RS421 library variant 13463
    Depositor
    Nili Ostrov
    Insert
    13463_[Tn5]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225792-plasmid-map-sequence-id-447013

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  9. pGLU_75

    Plasmid
    #225777
    Purpose
    RS421 library variant 13463
    Depositor
    Nili Ostrov
    Insert
    13463_[abr]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225777-plasmid-map-sequence-id-446990

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  10. pGLU_93

    Plasmid
    #225807
    Purpose
    RS421 library variant 13463
    Depositor
    Nili Ostrov
    Insert
    13463_[Himar]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225807-plasmid-map-sequence-id-446975

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  11. BTV

    Plasmid
    #131589
    Purpose
    Plasmid for the targeting of BAC clone
    Depositor
    Joon Tae Park
    Insert
    Firefly Luciferase
    Expression
    Mammalian
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    131589-plasmid-map-sequence-id-257086

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  14. pSB4A3-PBAD-phzO

    Plasmid
    #165619
    Purpose
    Arabinose-inducible expression of phzO for conversion of phenazine-1-carboxylic acid to 2-hydroxyphenazine
    Depositor
    Martin Buck
    Insert
    phzO
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    165619-plasmid-map-sequence-id-331406

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  15. pJK275

    Plasmid
    #73363
    Purpose
    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)
    Depositor
    T. J. Kappock
    Insert
    delta-8 sphingolipid desaturase
    Expression
    Yeast
    Mutation
    missing residues 1-6
    Promoter
    GAL1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73363-plasmid-map-sequence-id-138870

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  17. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    67921-plasmid-map-sequence-id-197668

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  23. pJK138

    Plasmid
    #73314
    Purpose
    Pas2p peroxisomal membrane targeting sequence
    Depositor
    T. J. Kappock
    Insert
    Pas2p (peroxisomal membrane targeting region)
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    encodes residues 1-42
    Promoter
    AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73314-plasmid-map-sequence-id-138890

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  24. pJK126

    Plasmid
    #73337
    Purpose
    Encodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)
    Depositor
    T. J. Kappock
    Insert
    electron transfer flavoprotein
    Tags
    His6
    Expression
    Bacterial
    Mutation
    encodes residues 328-685
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73337-plasmid-map-sequence-id-135048

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  25. pJK125

    Plasmid
    #73336
    Purpose
    Encodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)
    Depositor
    T. J. Kappock
    Insert
    electron transfer flavoprotein
    Tags
    His6
    Expression
    Bacterial
    Mutation
    encodes residues 1-327
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73336-plasmid-map-sequence-id-138202

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  31. pJK234

    Plasmid
    #73324
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC
    Depositor
    T. J. Kappock
    Inserts
    violacein biosynthesis protein VioA
    violacein biosynthesis protein VioB
    violacein biosynthesis protein VioC
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73324-plasmid-map-sequence-id-135032

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  36. pET28a-mxLOX1

    Plasmid
    #104975
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Insert
    MXAN_1745 (MXAN_1745 Myxococcus xanthus)
    Tags
    6 histidine tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    104975-plasmid-map-sequence-id-202893

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  37. pET28a-mxLOX2

    Plasmid
    #104976
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Insert
    MXAN_1744 (MXAN_1744 Myxococcus xanthus)
    Tags
    6 histidine tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    104976-plasmid-map-sequence-id-202892

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  41. INT_Cargo_T7_gutB

    Plasmid
    #212139
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    xylitol dehydrogenase
    Expression
    Bacterial
    Promoter
    PT7
    Available Since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212139-plasmid-map-sequence-id-424777

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  42. pET28a-mxEH

    Plasmid
    #104977
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Insert
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Tags
    6 histidine tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    104977-plasmid-map-sequence-id-202891

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  43. pJK139

    Plasmid
    #73315
    Purpose
    Pas2p peroxisomal membrane biogenesis protein
    Depositor
    T. J. Kappock
    Insert
    Pas2p peroxisomal membrane targeting protein
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    Encodes a glycine-204 to serine mutation
    Promoter
    AOX1
    Available Since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73315-plasmid-map-sequence-id-140431

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Showing: 951 - 1000 of 11308 results