We narrowed to 32,527 results for: Eng;

1. pCASCADE-udhA-xylA

   Plasmid

   #158612

   Purpose

   Low phosphate inducible gRNA array to silence the xylA and udhA promoters

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   udhA-xylA guide array
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCASCADE-gltA2-xylA

   Plasmid

   #158613

   Purpose

   Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   gltA2-xylA guide array
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCASCADE-zwf-xylA

   Plasmid

   #158614

   Purpose

   Low phosphate inducible gRNA array to silence the xylA and zwf promoters

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   zwf-xylA guide array
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZ_P-ICL1-eGFP

   Plasmid

   #126716

   Purpose

   yeast plasmid overexpressing eGFP

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   egfp
   

   Tags

   6xHis

   Expression

   Yeast

   Promoter

   ICL1

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDSM-de-Phhf2-PYC-Tadh1

   Plasmid

   #127737

   Purpose

   Yeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Phhf2

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZ_P-ACS1-eGFP

   Plasmid

   #126715

   Purpose

   yeast plasmid overexpressing eGFP

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   egfp
   

   Expression

   Yeast

   Promoter

   ACS1

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZ_P-ALD-eGFP

   Plasmid

   #126714

   Purpose

   yeast plasmid overexpressing eGFP

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   egfp
   

   Expression

   Yeast

   Promoter

   ALD

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZ_P-HXT1-FAS1up

   Plasmid

   #126746

   Purpose

   vector for replacing the promoter of FAS1 coding gene

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   fas1 upstream
   

   Tags

   6xHis

   Expression

   Yeast

   Promoter

   HXT1

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZ_P-HXT1-eGFP

   Plasmid

   #126719

   Purpose

   yeast plasmid overexpressing eGFP

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   egfp
   

   Expression

   Yeast

   Promoter

   HXT1

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZ_P-ADH3-eGFP

    Plasmid

    #126713

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Expression

    Yeast

    Promoter

    ADH3

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZ_P-GAP-ALD1

    Plasmid

    #126711

    Purpose

    yeast plasmid complementing ALD1 from Komagataella phaffii

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    ald1
    

    Tags

    6xHis,Myc

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZ_P-GAP-ADH3

    Plasmid

    #126710

    Purpose

    yeast plasmid complementing ADH3 from Komagataella phaffii

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh3
    

    Tags

    6xHis,Myc

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pK_lacO-cP-AOX1-sAR

    Plasmid

    #126743

    Purpose

    yeast plasmid overexpressing CPR and sLovA

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA,cpr
    

    Tags

    6xHis

    Expression

    Yeast

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDSM-bc-Prps3-CAD-Tcyc1

    Plasmid

    #127728

    Purpose

    Yeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Prps3

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDSM-bc-Peft2-CAD-Tklura3

    Plasmid

    #127729

    Purpose

    Yeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Peft2

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDSM-bc-Psmtef1-CAD-Tyhi9

    Plasmid

    #127730

    Purpose

    Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Psmtef1

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDSM-cd-Psptdh3-ACO-Tagtef1

    Plasmid

    #127734

    Purpose

    Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Psptdh3

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDSM-de-Ppgk1-PYC-Ttpi1

    Plasmid

    #127735

    Purpose

    Yeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Ppgk1

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDSM-de-Ppxr1-PYC-Tnat1

    Plasmid

    #127736

    Purpose

    Yeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Ppxr1

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDSM-ef-Psbtdh3-CSC-Tefm1

    Plasmid

    #127742

    Purpose

    Yeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    citrate synthase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Psbtdh3

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDSM-f3-Peno2-ACDH-Ttal1

    Plasmid

    #127743

    Purpose

    Yeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    acetylating acetaldehyde dehydrogenase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Peno2

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDSM-f3-Pvma6-ACDH-Trps14a

    Plasmid

    #127744

    Purpose

    Yeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    acetylating acetaldehyde dehydrogenase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Pvma6

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDSM-f3-Prpl28-ACDH-Tecm10

    Plasmid

    #127745

    Purpose

    Yeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    acetylating acetaldehyde dehydrogenase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Prpl28

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDSM-f3-Ptdh3-ACDH-Trpl3

    Plasmid

    #127746

    Purpose

    Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    acetylating acetaldehyde dehydrogenase
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Ptdh3

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. wtBxb1

    Plasmid

    #222337

    Purpose

    Plasmid for expressing wild type Bxb1 in mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wild type Bxb1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CMV-Nme2ABE8e

    Plasmid

    #189928

    Purpose

    Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    Nme2ABE8e
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CMV-CjABE8e

    Plasmid

    #189929

    Purpose

    Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    CjABE8e
    

    Expression

    Mammalian

    Mutation

    CjCas9 D8A

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAVS1 pegRNA1 with trimmed attP

    Plasmid

    #222346

    Purpose

    pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA1
    

    Use

    CRISPR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAVS1 pegRNA2 with trimmed attP

    Plasmid

    #222347

    Purpose

    pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA2
    

    Use

    CRISPR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. eeBxb1_IVT

    Plasmid

    #222342

    Purpose

    Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    Template for in vitro transcription

    Promoter

    T7 (inactivated)

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. aeBlue chromoprotein

    Plasmid

    #117846

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, aeBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. amajLime chromoprotein

    Plasmid

    #117843

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amajLime
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. fwYellow chromoprotein

    Plasmid

    #117841

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, fwYellow
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. tsPurple chromoprotein

    Plasmid

    #117848

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJBEI-6409

    Plasmid

    #47048

    Purpose

    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    

    Use

    Synthetic Biology; Bglbrick

    Expression

    Bacterial

    Promoter

    PlacUV5

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBS3Clux

    Plasmid

    #55172

    Purpose

    lux-reporter vector, integration at sacA, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. amilCP_Pink chromoprotein

    Plasmid

    #117851

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP_Pink
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    64A,65C, BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. amilCP_Orange chromoprotein

    Plasmid

    #117850

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP_Orange
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    64V,65G, BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. gfasPurple chromoprotein

    Plasmid

    #117849

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, gfasPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. CMV-SauriABE8e

    Plasmid

    #189927

    Purpose

    Plasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SauriABE8e
    

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. spisPink chromoprotein

    Plasmid

    #117839

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, spisPink
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. asPink chromoprotein

    Plasmid

    #117838

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, asPink
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. scOrange chromoprotein

    Plasmid

    #117840

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, scOrange
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBS2E

    Plasmid

    #55169

    Purpose

    Empty vector, integration at lacA, ampr, mlsr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

47. eforRed chromoprotein

    Plasmid

    #117837

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, eforRed
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pJBEI-6410

    Plasmid

    #47049

    Purpose

    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    

    Use

    Synthetic Biology; Bglbrick

    Expression

    Bacterial

    Promoter

    PlacUV5

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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49. pBS4S

    Plasmid

    #55170

    Purpose

    Empty vector, integration at thrC, ampr, specr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

50. pCA24N-ligase-NLS-cTF

    Plasmid

    #87746

    Purpose

    IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and NLS linker - cTF (chimeric transcriptio…

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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