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Addgene

We narrowed to 19,977 results for: ACE;

Showing: 381 - 400 of 19977 results
  1. AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR

    Plasmid
    #190079
    Purpose
    express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene
    Depositor
    Insert
    ACE2 (TMPRSS2 Rhinolophus alcyone and Rhinolophus ferrumequinum, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    none
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

    Plasmid
    #49080
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C791S, C820S, C1052S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Nov. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
  3. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

    Plasmid
    #214138
    Purpose
    Insect cell expression of human 20S CP alpha-subunits
    Depositor
    Inserts
    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    PSMA5 (PSMA5 Human)
    PSMA6 (PSMA6 Human)
    PSMA7 (PSMA7 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

    Plasmid
    #176589
    Purpose
    Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer
    Depositor
    Insert
    CRISPR-associate IscB (Chesapeake Bay) locus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Truncated CRISPR array to two direct repeats, rep…
    Promoter
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid
    #172501
    Purpose
    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers
    Insert
    DisCas7-11 full locus with Golden gate acceptor site for spacer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid
    #172908
    Purpose
    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
    Depositor
    Has Service
    AAV1
    Insert
    Ace2N-4AA-mNeon-ST A122D
    Use
    AAV and Cre/Lox
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    hSynapsin1
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid
    #197031
    Purpose
    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter
    Depositor
    Insert
    humanized denOsCas12f1-VPR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7

    Plasmid
    #214140
    Purpose
    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7
    Depositor
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid
    #214139
    Purpose
    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2
    Depositor
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. AiP14002 - pAAV-AiE0064h-spacer-AiE0022h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4002)

    Plasmid
    #230389
    Purpose
    AiE0064h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minBG
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. EW375 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid
    #236177
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and GFP-NZF dummy transcription factor
    Depositor
    Inserts
    EGFP-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/IKZF1 sites
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. EW373 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid
    #236175
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and GFP-NZF dummy transcription factor
    Depositor
    Inserts
    EGFP-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF250 sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. EW374 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid
    #236176
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and GFP-NZF dummy transcription factor
    Depositor
    Inserts
    EGFP-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF35 sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176459
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Insert
    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 381 - 400 of 19977 results