Addgene

We narrowed to 4,147 results for: Bre;

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pcDNA5-FRT-TO-N-Flag-8His

Plasmid

#157677

Purpose

N-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Flag-8His

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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STAGR_gRNAScaffold_hU6

Plasmid

#102840

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Use

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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alpha7nACHR_Nacho_mCherry

Plasmid

#182939

Purpose

alpha7 nACHR expression in mammalian cell lines

Depositor

Robert Brenner

Article

Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

Insert

NACH7 (CHRNA7 Human)

Use

Synthesized

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV: Flag-Antares_SV40:PuroR

Plasmid

#183049

Purpose

Expresses FLAG-tagged Antares in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

Antares BRET reporter

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

Plasmid

#188703

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP

Plasmid

#157733

Purpose

mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-IETD-NanoLuc

Plasmid

#98288

Purpose

caspase-8 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F0

Plasmid

#184165

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-UBE2S-UBD

Plasmid

#66713

Purpose

Expresses the UBE2S-UBD fusion protein in E. coli

Depositor

David Komander

Article

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Inserts

e2-epf (UBE2S )
isot (USP5 )

Use

Tags

GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-C-8His-Flag

Plasmid

#157678

Purpose

C-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

8His-Flag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMC_mClover3_BSDminus_F2

Plasmid

#184167

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F1

Plasmid

#184166

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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SeeSaw Reporter (SSR1.0)

Plasmid

#50392

Purpose

SSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.

Depositor

Pablo Huertas Sánchez

Article

Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

Insert

SSR1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pMB22

Plasmid

#122244

Purpose

Lentivirus delivery for stable expression of AsCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Expression

Mutation

Promoter

EFs

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mPea3 FL (CT#1119)

Plasmid

#13908

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Pea3 (Etv4 Mouse)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-RaACR_687-EYFP

Plasmid

#111077

Purpose

Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RaACR_687

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_h7SK

Plasmid

#102842

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_h7SKpromoter

Use

As pcr template

Tags

Expression

Mutation

Promoter

h7SK

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pUAST cyto roGFP2-Orp1

Plasmid

#64996

Purpose

for expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Use

Tags

roGFP2

Expression

Insect

Mutation

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pVAX-Help-SIINFEKL

Plasmid

#141350

Purpose

DNA vaccination plasmid containing SIINFEKL and several CD4+ T cell epitopes

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

HELP-SIINFEKL-KDEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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