We narrowed to 24,857 results for: Nes;

1. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

   Plasmid

   #194685

   Purpose

   CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_09-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

   Plasmid

   #194686

   Purpose

   CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

   Plasmid

   #194687

   Purpose

   CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNESx2-EGFP-C1-PABDx2-Spo20

   Plasmid

   #220082

   Purpose

   PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   Spo20 (SPO20 Budding Yeast)
   

   Use

   Tags

   X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

   Expression

   Mammalian

   Mutation

   amino acids 51-91, tandem dimer

   Promoter

   CMV

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-NES-ZapCV2-Zinc dead

   Plasmid

   #203850

   Purpose

   Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

   Depositor

   Amy Palmer

   Article

   Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

   Insert

   ZapCV2-Ala zinc dead sensor
   

   Use

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   Cys and His in Zap1 is mutated with Ala

   Promoter

   CMV promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-HyPer-DAAO-NES

   Plasmid

   #119164

   Purpose

   Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.

   Depositor

   Thomas Michel

   Article

   Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

   Insert

   HyPer-DAAO-NES
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB1679 CMV-HIVNES-GS-Cas13bt3

   Plasmid

   #176317

   Purpose

   CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Insert

   human codon optimized Cas13bt3
   

   Use

   CRISPR

   Tags

   HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CAG-DIO-APEX2NES

   Plasmid

   #79907

   Purpose

   Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

   Depositor

   Joshua Sanes

   Article

   Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

   Insert

   APEX2NES
   

   Use

   AAV and Cre/Lox

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR_L1-YFP-miniTurbo-NES-L2

   Plasmid

   #127351

   Purpose

   gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBpuro-4493NES(Booster-PKA)

    Plasmid

    #184709

    Purpose

    PKA biosensor

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    Booster-PKA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid

    #127369

    Purpose

    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV gfaABC1D NES-jRCaMP1a

    Plasmid

    #171120

    Purpose

    Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    His Tag, T7 Tag, XpressTag

    Expression

    Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmBanana-Hyg-CTCF-CtermNES-N1

    Plasmid

    #131851

    Purpose

    Expresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cells

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    Tags

    mBanana

    Expression

    Mammalian

    Mutation

    the C-terminus of CTCF is linked to HIV-Rev prote…

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

    Plasmid

    #194690

    Purpose

    hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

    Plasmid

    #194691

    Purpose

    hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV_CAG_V5-LOV-Turbo-NES

    Plasmid

    #199670

    Purpose

    expresses V5-LOV-Turbo-NES in mammalian cells, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    AAV

    Tags

    NES and V5

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

    Plasmid

    #194692

    Purpose

    hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-SypHer2-DAAO-NES

    Plasmid

    #119184

    Purpose

    Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

    Depositor

    Thomas Michel

    Article

    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

    Insert

    SypHer2-DAAO-NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR_L1-YFP-Turbo-NES-L2

    Plasmid

    #127349

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKII-iGECI-NES

    Plasmid

    #160424

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI-NES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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