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Showing: 381 - 400 of 13577 results

  1. pBCC096

    Plasmid
    #202222
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC30, pBCC57, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202222-plasmid-map-sequence-id-403461

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  2. pBCC097

    Plasmid
    #202223
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and tagBFP fluorescent marker
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC30, pBCC59, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202223-plasmid-map-sequence-id-403473

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  3. pLY237

    Plasmid
    #184150
    Purpose
    Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Insert
    s-tracrRNA-R1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD promoter
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184150-plasmid-map-sequence-id-376095

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  4. pLY251

    Plasmid
    #184153
    Purpose
    Reprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Insert
    s-tracrRNA-Ar2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD promoter
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184153-plasmid-map-sequence-id-376090

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  6. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Inserts
    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

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  7. pRMC2-msfGFP

    Plasmid
    #194913
    Purpose
    Tetracycline inducible expression of msfGFP in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194913-plasmid-map-sequence-id-384624

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  8. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

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  9. pIN4_tsp-6_mScarlet

    Plasmid
    #182935
    Purpose
    Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)
    Depositor
    Maureen Barr
    Article
    Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6
    Insert
    Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)
    Use
    Tags
    flexible linker (GGGGS)x3 and mScarlet tag
    Expression
    Worm
    Mutation
    Promoter
    tsp-6
    Available since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    182935-plasmid-map-sequence-id-368447

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  10. pUASTLOTattB_mCherry-CD8-vhhGFP

    Plasmid
    #163930
    Purpose
    Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Article
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163930-plasmid-map-sequence-id-323460

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  13. pBCC091

    Plasmid
    #202218
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC30, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202218-plasmid-map-sequence-id-403465

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  15. Cancer Pathways ORFs Kit

    Plasmid Kit
    #1000000072
    Purpose
    A set of vectors containing cDNAs for studying oncogenic signaling pathways. cDNAs are either wild-type, constitutively active mutants, or dominant negative mutants.
    Depositor
    David Sabatini, Kris Wood
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. Sema6b(S)-AP-His

    Plasmid
    #72040
    Purpose
    Expresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Sema6b (Sema6b Mouse)
    Use
    Tags
    AP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72040-plasmid-map-sequence-id-131226

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  18. pcS-E626-C1C2

    Plasmid
    #200162
    Purpose
    This plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.
    Depositor
    Masako Harada
    Article
    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)
    Inserts
    E626
    C1C2 domain of lactadherin
    Use
    Tags
    HA and HIS
    Expression
    Mammalian
    Mutation
    sequence adopted from doi: 10.1371/journal.pone.0…
    Promoter
    CMV
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200162-plasmid-map-sequence-id-396334

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  19. pRMC2-tat:msfGFP

    Plasmid
    #194915
    Purpose
    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194915-plasmid-map-sequence-id-384626

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  20. pRMC2-sec:msfGFP

    Plasmid
    #194914
    Purpose
    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194914-plasmid-map-sequence-id-384625

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Showing: 381 - 400 of 13577 results