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pHEN-CoV2-nanobody-F1

Plasmid

#194592

Purpose

Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

F1 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_nLightR

Plasmid

#187180

Purpose

Expresses red norepinephrine indicator nLightR in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has Service

AAV9

Insert

nLightR

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-COV E

Plasmid

#165123

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

COV E (E codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-COV M

Plasmid

#165124

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

COV M (M codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ-ABE-Cas9n-Act3.0

Plasmid

#178956

Purpose

It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pME map2k2a_s219D

Plasmid

#201058

Purpose

Gateway middle entry vector expressing constitutively active zebrafish map2k2a

Depositor

Sarah Childs

Article

Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

Insert

MAP2k2a (map2k2a Zebrafish)

Use

Zebrafish expression

Mutation

Mutation S219D, constitutively active

Promoter

none

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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V2R-GFP

Plasmid

#208910

Purpose

encodes the human vasopressin receptor 2, tagged with GFP

Depositor

Christian Gruber

Article

Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

Insert

AVPR2 (AVPR2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-TEX264-deltaLRR-His6

Plasmid

#220215

Purpose

Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

His6

Expression

Bacterial

Mutation

c.4-75del / p.2-24del

Promoter

T7

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-SYP1-miniSOG-citrine

Plasmid

#50971

Purpose

AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

Synaptophysin-miniSOG-citrine (Syp Rat)

Use

AAV

Tags

citrine and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK1

Plasmid

#138692

Purpose

Expresses a human NUAK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK1 human (NUAK1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-C7

Plasmid

#194591

Purpose

Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

C7 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ORF9b

Plasmid

#165122

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF9b

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF2

Plasmid

#200385

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-SYP1-miniSOG-T2A-mCherry

Plasmid

#50972

Purpose

AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Has Service

AAV8

Insert

Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)

Use

AAV

Tags

T2A-mCherry and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

Plasmid

#214733

Purpose

Expresses mCherry protein in infected and cre positive cells

Depositor

Félix Leroy

Article

de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

Insert

anti-Crh shRNA (scrambled) / mCherry

Use

AAV

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

Plasmid

#214732

Purpose

Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

Depositor

Félix Leroy

Article

de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

Insert

anti-Crh shRNA / mCherry

Use

AAV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK2

Plasmid

#138693

Purpose

Expresses a human NUAK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK2 human (NUAK2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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PHB2 S39D-mCherry

Plasmid

#232787

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Changed Serine 39 to Aspartate

Promoter

CMV

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Tbx20

Plasmid

#72697

Purpose

Doxycyline regulated expression of Tbx20 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Tbx20 (Tbx20 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304 3xFlag-eRF1_IRES-GFP

Plasmid

#198393

Purpose

eRF1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eRF1 (ETF1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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