167,565 results

1. lenti-EF1a-dCas9-VPR-Puro

   Plasmid

   #99373

   Purpose

   3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   (Sp)dCas9-VPR-P2A-Puro
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-LAMP1-mCherry

   Plasmid

   #214274

   Purpose

   Mammalian expression of LAMP1-mCherry (lysosome marker)

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   LAMP1-mCherry (Lamp1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

   Plasmid

   #108833

   Purpose

   Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment aa28 to aa396
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS and GAI

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

   Plasmid

   #176027

   Purpose

   construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   rtTA3
   Luc2
   mtagBFP
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pExp-His-zBasic-RBD

   Plasmid

   #194999

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli

   Depositor

   Marko Hyvönen

   Article

   Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

   Insert

   RBD (S SARS-CoV-2)
   

   Tags

   His8-Zbasic

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RF-118-UB-BRCA1

   Plasmid

   #78960

   Purpose

   Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells

   Depositor

   Richard Fahlman

   Article

   Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   3x Flag and ubiquitin

   Expression

   Mammalian

   Mutation

   delta 1-1155

   Promoter

   CMV

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNP3254 [pNP2922 - ISCro4 Enhanced DBL (DBL3, WT) + ISCro4 Recombianse (WT]

   Plasmid

   #247257

   Purpose

   Express ISCro4 recombinase and bridge RNA

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Insert

   pNP2922 - ISCro4 bRNA101-179 ins179(rc(101-111) (DBL3)
   

   Expression

   Mammalian

   Mutation

   ins179(rc(101-111)

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNP3386 [For use in genome insertion and off-target assessment]

   Plasmid

   #247258

   Purpose

   Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Insert

   ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pvPE-V2

   Plasmid

   #240286

   Purpose

   Encodes second generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

   Depositor

   Lusheng Huang

   Article

   Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

   Insert

   pvPE-V2
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pvPE-V3

    Plasmid

    #240287

    Purpose

    Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.

    Depositor

    Lusheng Huang

    Article

    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

    Insert

    pvPE-V3
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge