We narrowed to 30,517 results for: Ide

1. pET-FLAG-SpCas9-HF1

   Plasmid

   #126770

   Purpose

   Expression of increased fidelity SpCas9-HF1 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS Galectin1

   Plasmid

   #19216

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   Galectin1 (LGALS1 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pPI304

   Plasmid

   #113232

   Purpose

   extrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFP

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   LifeAct & pkgE-PH
   

   Use

   Dictyostelium discoideum expression vector, extra…

   Tags

   mCherry & GFP

   Expression

   Mutation

   Promoter

   act15

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAdSh.U6.gRNAGFP

   Plasmid

   #58255

   Purpose

   U6 promoter-driven single guide RNA complementary to eGFP

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAGFP cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. A3Bmax

   Plasmid

   #207167

   Purpose

   Express full-length human A3B in a BE4max-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
   

   Use

   Tags

   NLS and NLS, 6x His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-Sox17

   Plasmid

   #60669

   Purpose

   PiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Sox17 (Sox17 Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL0426 (CRISPR with spc3)

   Plasmid

   #130642

   Purpose

   Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   CRISPR(spacer-3)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLO2 casX

   Plasmid

   #123122

   Purpose

   E. coli expression vector for CasX sgRNA

   Depositor

   Jennifer Doudna, Benjamin Oakes

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   CasX guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAdSh.U6.gRNAS1

   Plasmid

   #58252

   Purpose

   U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAS1 cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA scaffold-spacer-human U6

    Plasmid

    #214697

    Purpose

    provide "sgRNA scaffold-spacer-human U6" fragment

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    sgRNA scaffold-spacer-human U6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUASt-GBP-Trbo

    Plasmid

    #179996

    Purpose

    Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.

    Depositor

    Graydon Gonsalvez

    Article

    Baker et al Development. 2021 Nov 15;148(22). pii: 2

    Insert

    GBP nanobody
    

    Use

    Tags

    Trbo

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCold I-Hero9

    Plasmid

    #187927

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero9

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero9
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDUAL_2_T5

    Plasmid

    #155175

    Purpose

    see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBR322-bom

    Plasmid

    #155178

    Purpose

    pBICI_2_strong without bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTL-Bag3-gRNA3

    Plasmid

    #196254

    Purpose

    Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTL-Bag2-gRNA2

    Plasmid

    #196252

    Purpose

    Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTL-Bag1-gRNA1

    Plasmid

    #196251

    Purpose

    Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTL-Bag4-gRNA4

    Plasmid

    #196255

    Purpose

    Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTL-Bag2T-gRNA2

    Plasmid

    #196253

    Purpose

    Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDT-sgRNA-XMAS-2x

    Plasmid

    #164414

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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