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Plasmid#63629PurposeK1F-R5-IRH RNAP mutantDepositorInsertKIF-R5-IRH RNAP
UseTagsExpressionMutationL749I, Q754S, R756N, I761V, H772R, and Q786HPromoterT5/LacAvailable sinceJune 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pfbiRS
Plasmid#67283PurposeExpresses the fbiC gene from Mycobacterium smegmatisDepositorInsertfbiC gene from Mycobacterium smegmatis
UseTagspolyhistidineExpressionMammalianMutationnon knownPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
NK641
Plasmid#109100PurposeReporter gene expression in S. rosettaDepositorInsertPTSG_07215
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK601
Plasmid#109070PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_03784
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY4
Plasmid#21763DepositorInsertECFP -EYFP linked by flexible linker containing 4 SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
NK590
Plasmid#109084PurposeReporter gene expression in S. rosettaDepositorInsertmPapaya
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNJB80
Plasmid#51518PurposeGateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
NK594
Plasmid#109085PurposeReporter gene expression in S. rosettaDepositorInsertTagRFP-T
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3C06
Plasmid#70979PurposeGateway entry cloneDepositorInsertCG9246
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
NK649
Plasmid#109105PurposeReporter gene expression in S. rosettaDepositorInsertPTSG_06009
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3D11
Plasmid#71007PurposeGateway entry cloneDepositorInsertAcf1
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSKB3.TM1777
Plasmid#11421DepositorInsertNusA (nusA T. maritima)
UseTags6x His and TEV protease siteExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_5'_Linker
Plasmid#49291PurposeGolden Gate entry vector at position 5, small linker insert, final entry vector for the assemblyDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Phelo
Plasmid#48995PurposeGateway adapted transfer of Phleomycin resistance geneDepositorInsertSh ble
UseGateway attb1 & attb2TagsExpressionMutationPromoterAvailable sinceApril 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28CLY8
Plasmid#21767DepositorInsertECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYZ182
Plasmid#98424PurposePombe Expression Vector - nmt1 promoter with empty BsaI-pad - no markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ189
Plasmid#98427PurposePombe General Purpose Vector - with empty BsaI-pad for new potential markersDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_4'_XcmI-LacZ
Plasmid#49305PurposeGolden Gate entry vector at position 4, LacZ, final entry vector for the assemblyDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4G02
Plasmid#71025PurposeGateway entry cloneDepositorInsertCG3711
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
NK619
Plasmid#109089PurposeReporter gene expression in S. rosettaDepositorInsertmCherry
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only