We narrowed to 9,920 results for: 158

1. pAPM-miR30-IFIH1-ts3

   Plasmid

   #174261

   Purpose

   IFIH1 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   IFIH1 shRNA (IFIH1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-MCP-huADARE488QT490A

   Plasmid

   #159922

   Purpose

   Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

   Depositor

   Fei Chen

   Article

   Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

   Insert

   Human ADAR2 E488QT490A (ADARB1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCbs FlagOmomyc

   Plasmid

   #113168

   Purpose

   Expression of FLAG tagged Omomyc in mammalian cells

   Depositor

   Sergio Nasi

   Article

   Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

   Insert

   Omomyc (MYC Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E57T, E64I, R70Q, R71N

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304-FOS-V5

   Plasmid

   #59140

   Purpose

   wild-type FOS with C-terminal V5 tag expressed from pLX vector

   Depositor

   William Hahn

   Article

   Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

   Insert

   FOS (FOS Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-eNme2-T.2-ABE8e

   Plasmid

   #185669

   Purpose

   Expresses eNme2-T.2-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-T.2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/Myc-His beta4

   Plasmid

   #16039

   Depositor

   Filippo Giancotti

   Article

   Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

   Insert

   beta4 (ITGB4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

   Plasmid

   #114067

   Purpose

   T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   entry vector for AsCas12a crRNAs
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5-LAP-MPS1-WT

   Plasmid

   #114044

   Purpose

   Expression of exogenous LAP tagged MPS1 WT

   Depositor

   Geert Kops

   Article

   Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

   Insert

   MPS1-WT (TTK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT MPS1 sequence

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2108

    Plasmid

    #143338

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    L3MBTL4 (L3MBTL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MC2R-Tango

    Plasmid

    #66428

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MC2R (MC2R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRS0045

    Plasmid

    #131124

    Purpose

    Mammalian Target-ACE expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1, TadA, and UGI

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-YAP-wt

    Plasmid

    #229412

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    YAP-wt (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCLXEBR-pTF-cKit

    Plasmid

    #114293

    Purpose

    TET-inducible expression of c-Kit

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    cKit (Kit Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b(+)_rPD-L1nb

    Plasmid

    #226715

    Purpose

    Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag and Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH-nCas9-PPE-V2

    Plasmid

    #170131

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

    Insert

    nCas9(H840A)-M-MLV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A for Cas9; D200N, T306K, W313F, T330P and L…

    Promoter

    maize Ubiquitin-1, OsU3

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-EIN-AlkBH5(66-292)

    Plasmid

    #125732

    Purpose

    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    AlkBH5(66-292) (ALKBH5 Human)
    

    Use

    Tags

    6xHis and EIN

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30a-His6-ABP-LOXCAT

    Plasmid

    #139971

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Circular 200,100 (mPCSK9)

    Plasmid

    #170122

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Pcsk9 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWZ853

    Plasmid

    #208115

    Purpose

    mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSD

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mNeonGreen-HPRT1-BSD
    

    Use

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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