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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 31,534 results for: Eng

Showing: 6091 - 6120 of 31534 results

  1. pKan cpRBP-FL P53

    Plasmid
    #174504
    Purpose
    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
    Depositor
    Stewart Loh
    Article
    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do
    Insert
    P53 gene (TP53 Human)
    Use
    Tags
    20 amino acid linker containing HRV3C protease si…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174504-plasmid-map-sequence-id-344828

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  2. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

    Plasmid
    #176334
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617.2 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19R, G142D, del156-157, R158G, L452R, T478K, D61…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176334-plasmid-map-sequence-id-347146

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  4. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

    Plasmid
    #184533
    Purpose
    Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-BA.2 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    184533-plasmid-map-sequence-id-361466

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  6. pQhsCDtRNA+

    Plasmid
    #86014
    Purpose
    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    LPP and Ptac
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86014-plasmid-map-sequence-id-169485

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  7. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-T478K-AVI

    Plasmid
    #176456
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-L452R-T478K (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    L452R, T478K
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176456-plasmid-map-sequence-id-347373

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  8. pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

    Plasmid
    #176325
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.7 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176325-plasmid-map-sequence-id-347062

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  9. CCR5-SZ190b-sfGFP

    Plasmid
    #162447
    Purpose
    Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration
    Depositor
    Shuguang Zhang
    Article
    Qing et al iScience. 2020 Oct 28;23(12):101670. doi
    Insert
    C-C chemokine receptor type 5 (CCR5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    truncation from aa88 to aa249
    Promoter
    CMV
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    162447-plasmid-map-sequence-id-331353

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  11. pVRC8400-SARS-CoV-2-S2P-B.1.351-AVI

    Plasmid
    #176326
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.351 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, 242-244del, R246I, K417N, E484…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176326-plasmid-map-sequence-id-347063

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  13. pVRC8400-SARS-CoV-2-S2P-P.1-AVI

    Plasmid
    #176327
    Purpose
    Expresses SARS-CoV-2 S2P protein from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-P.1 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176327-plasmid-map-sequence-id-347067

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  14. pZE21/UBP1/ClpS_V65I

    Plasmid
    #98567
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Contains the V65I mutation that increases discrim…
    Promoter
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98567-plasmid-map-sequence-id-203054

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  20. pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122

    Plasmid
    #223184
    Purpose
    Plasmid containing the Zfr/Firefly luciferase/3xMIR122 cassette. Translation of luciferase is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.
    Depositor
    Yuxuan Guo
    Article
    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10
    Inserts
    luciferase (LOC116160065 firefly)
    mir122 (Mir122 Mouse)
    Use
    AAV
    Tags
    HA and engineered P2A
    Expression
    Mutation
    Promoter
    TNNT2
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    223184-plasmid-map-sequence-id-443757

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  23. pML624_RAB1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227906
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RAB1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    227906-plasmid-map-sequence-id-458972

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  27. pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227923
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RPL36 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227923-plasmid-map-sequence-id-456037

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  28. pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227925
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227925-plasmid-map-sequence-id-456028

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  29. pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227926
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227926-plasmid-map-sequence-id-452495

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  30. pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227908
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227908-plasmid-map-sequence-id-452488

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Showing: 6091 - 6120 of 31534 results