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LI F-G Gent

Plasmid

#104522

Purpose

LI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Apramycin resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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p236_LTJ_sgRNAFoxp3sf/J

Plasmid

#82676

Purpose

sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 sf/J

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS4-IVS-Syn21-GFP-P10

Plasmid

#104615

Purpose

Expresses GFP upon binding of TALE4 to VAS4

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS4

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE-VP64-P10

Plasmid

#104605

Purpose

Control plasmid (no TALE array) with P10 terminator

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Type

Empty backbone

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3_zfh1_CP-candidate_luc+

Plasmid

#86391

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-Tag/S46X with 5'UTR

Plasmid

#87729

Purpose

Expresses S46X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S46X

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S44L with 5'UTR

Plasmid

#87727

Purpose

Expresses S44L spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S44L

Promoter

pCMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/E112K with 5'UTR

Plasmid

#87723

Purpose

Expresses E112K spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

E112K

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/L195V with 5'UTR

Plasmid

#87725

Purpose

Expresses L195V spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

L195V

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/myc-M1E112K

Plasmid

#87715

Purpose

Expresses myc-M1E112K spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; E112K

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-Pl-lacO1-deGFP-T500 (123p)

Plasmid

#70199

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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AEB-T4-2

Plasmid

#63855

Purpose

This pFTV1 vector contains a thyroxine binding riboswitch, controlling the translation initaition rate of luciferase gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

T4-2 thyroxine riboswitch

Use

Synthetic Biology

Promoter

tac

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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AEB-TMR7pUC19

Plasmid

#63857

Purpose

This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

TMR7 TMR riboswitch

Use

Synthetic Biology

Promoter

tac

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pBEST-p15A-J23113-deGFP-T500 (124p)

Plasmid

#70200

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-pNull-deGFP-T500 (133p)

Plasmid

#70209

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-J23102-deGFP-T500 (132p)

Plasmid

#70208

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-J23100-deGFP-T500 (130p)

Plasmid

#70206

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-J23151-deGFP-T500 (129p)

Plasmid

#70205

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-J23106-deGFP-T500 (128p)

Plasmid

#70204

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-J23150-deGFP-T500 (127p)

Plasmid

#70203

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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