We narrowed to 17,783 results for: ada

1. 6xHis-MBP-huErCas12a

   Plasmid

   #174685

   Purpose

   Expresses 6xHis-MBP tagged ErCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huErCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis

   Expression

   Bacterial

   Mutation

   Q926R mutation is observed as indicated from the …

   Promoter

   T7

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 V5 hHR23A

   Plasmid

   #13053

   Depositor

   Steven Grossman

   Article

   Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

   Insert

   hHR23A (RAD23A Human)
   

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   R325K and L355P relative to NP_001257291.1

   Available Since

   Oct. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. EcNusGFL

   Plasmid

   #113122

   Purpose

   Expresses full-length E. coli NusG

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   NusG (nusG Escherichia coli)
   

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. px459 VQR

   Plasmid

   #101715

   Purpose

   sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q and T1337R)

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFAP-Arclight

   Plasmid

   #187978

   Purpose

   Expresses the fluorescent voltage indicator Arclight under the GFAP promoter

   Depositor

   Chris Dulla

   Article

   Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

   Insert

   Arclight A242
   

   Use

   AAV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSTAR-mOrange2-sfGFP

   Plasmid

   #115693

   Purpose

   Generation of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent Timer

   Depositor

   David Suter

   Article

   Alber et al Mol Cell. 2018 Aug 17. pii: S1097-2765(1

   Insert

   SA-mOrange2-sfGFP-SD
   

   Use

   Lentiviral

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-puro-Flag-mMcl-1

   Plasmid

   #32982

   Depositor

   Joseph Opferman

   Article

   Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

   Insert

   Mcl-1 (Mcl1 Mouse)
   

   Use

   Retroviral

   Tags

   3XFlag

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pRS415_pGal-nCas9(D10A)

   Plasmid

   #79618

   Purpose

   Expresses nCas9(D10A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAID1.2-EF1a-NmScarlet-mAID

   Plasmid

   #140617

   Purpose

   Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Expression

   Mammalian

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-TFAP4

    Plasmid

    #110156

    Purpose

    Expression of human TFAP4 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Yellow Cameleon-Nano15/pcDNA3

    Plasmid

    #51961

    Purpose

    mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Expression

    Mammalian

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. psiCHECK2-2A-3xFLAG-SBP-2A

    Plasmid

    #159584

    Purpose

    Luciferase reporter for ribosome pausing control

    Depositor

    Shintaro Iwasaki

    Article

    Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

    Inserts

    2A-3xFLAG-SBP-2A
    EIF2S3 5UTR
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTarget

    Plasmid

    #186452

    Purpose

    used as source for acquisition of new spacers

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Clathrin-LCa-EYFP

    Plasmid

    #21741

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

15. BirA-Myc_N_TAL1

    Plasmid

    #167728

    Purpose

    Expresses TAL1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    TAL1 (TAL1 Human)
    

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMA3288

    Plasmid

    #46885

    Purpose

    Lentiviral vector encoding Tet-inducible mitochondrially targeted Wild Type human Uracil-N-glycosylase

    Depositor

    Mikhail Alexeyev

    Article

    Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

    Insert

    Uracil-N-glycosylase WT (UNG Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    TRE-Tight

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPMVAu8

    Plasmid

    #98297

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
    

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFLAG-ZYG11B

    Plasmid

    #110550

    Purpose

    expresses a FLAG-tagged human ZYG11B protein in mamalian cells

    Depositor

    Edward Kipreos

    Article

    Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

    Insert

    ZYG11B (ZYG11B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. ITPKA_3xmNeonGreen

    Plasmid

    #129606

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    F-tractin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAID1.2-EF1a-CmCherry-mAID

    Plasmid

    #140604

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Expression

    Mammalian

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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