We narrowed to 11,761 results for: pcDNA

1. pcDNA5-FRT-TOgw-C-mCherry

   Plasmid

   #223952

   Purpose

   Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CibN-dCas9-CibN

   Plasmid

   #60553

   Purpose

   Expresses CibN-dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-HIF2alpha-P405A/P531A-pcDNA3

   Plasmid

   #18956

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1- dCas9-2xNLS-EGFP

   Plasmid

   #74710

   Purpose

   Expresses dCas9-2xNLS-EGFP in mammalian cells

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   dCas9-2xNLS-EGFP
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

   Plasmid

   #115788

   Purpose

   Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   LRP5 (LRP5 Human)
   

   Use

   Tags

   3xflag

   Expression

   Mammalian

   Mutation

   contains silent mutation that creates an internal…

   Promoter

   CMV

   Available since

   Oct. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-MBP1-E322TAG-W340A.pcDNA3-k

   Plasmid

   #127404

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: E322 to Amber codon and W34…

   Promoter

   CMV

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/opto-a1AR-EYFP

   Plasmid

   #20947

   Purpose

   Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

   Depositor

   Karl Deisseroth

   Article

   Airan et al Nature. 2009 Mar 18. ():.

   Insert

   opto-a1AR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA-FLAG-METTL3-APPA

   Plasmid

   #160251

   Purpose

   Express catalytic inactive FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   CMV

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-HNF1A-WT-Flag-UTR

   Plasmid

   #183237

   Purpose

   Expression of FLAG tagged HNF1A

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   HNF1 homeobox A (HNF1A Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-NES-PhoCl2f-mCherry

    Plasmid

    #164048

    Purpose

    Optogenetic control of protein localization by PhoCl2f

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-PhoCl2f-mCherry
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_miniSOG2 T2A H2B-EGFP

    Plasmid

    #87410

    Purpose

    Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

    Depositor

    Xiaokun Shu

    Article

    Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

    Insert

    mini singlet oxygen generator 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4-TO-Hygromycin-sfGFP-MAP

    Plasmid

    #44100

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Use

    Tags

    sfGFP-MAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1 AURKA Lys162Met

    Plasmid

    #157751

    Purpose

    Expression of kinase-dead AURKA K62M in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-FLAG-Dicer-delHelicC

    Plasmid

    #51366

    Depositor

    Narry Kim

    Article

    Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

    Insert

    Dicer (DICER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    From N terminus to HelicC domain deletion

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Yellow Cameleon-Nano65/pcDNA3

    Plasmid

    #51965

    Purpose

    mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1+(Zeo)-FLAG-muGFP

    Plasmid

    #166726

    Purpose

    Expresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cells

    Depositor

    Daniel Scott

    Article

    Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

    Insert

    muGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-FLAG-human NPM1 wt

    Plasmid

    #160692

    Purpose

    Mammalian expression of FLAG-tagged NPM1

    Depositor

    Charles Sherr

    Article

    Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1+ hnRNPK myc HIS

    Plasmid

    #71662

    Purpose

    Mammalian expression of hnRNPK

    Depositor

    Gideon Dreyfuss

    Insert

    hnRNPK (HNRNPK Human)
    

    Use

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO GFP DNAJB1

    Plasmid

    #19495

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB1 (DNAJB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-FLAG-Dicer-delPAZ

    Plasmid

    #51367

    Depositor

    Narry Kim

    Article

    Lee et al EMBO J. 2006 Feb 8;25(3):522-32. Epub 20

    Insert

    Dicer (DICER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    From N terminus to PAZ domain deletion

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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